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Peer-reviewedCC BY 4.0Biomedical optics express

Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates

  • Charlie Kersuzan
  • Anirban Jana
  • Amaury Badon
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Abstract

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method that eliminates piezoelectric displacement and synchronization by continuously translating the sample with a motorized stage while recording images on the fly. Depth-resolved information is retrieved via Fourier analysis of the temporal signal at each pixel. This approach enables volumetric imaging over several hundred micrometers within tens of seconds and provides a higher contrast-to-noise ratio than traditional four-phase FF-OCT. Continuous-scanning FF-OCT thus represents a simpler and faster alternative for 3D bio-imaging of living tissues and organoids.

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Cite

BibTeXRIS
BibTeX
@article{kersuzan2026continuous,
  title = {{Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates}},
  author = {Charlie Kersuzan and Anirban Jana and Amaury Badon},
  journal = {Biomedical optics express},
  year = {2026},
  month = oct,
  doi = {10.1364/boe.578177},
  url = {https://hal.science/hal-05347526v2},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates
AU  - Charlie Kersuzan
AU  - Anirban Jana
AU  - Amaury Badon
PY  - 2026
DA  - 2026/10/09
JO  - Biomedical optics express
DO  - 10.1364/boe.578177
UR  - https://hal.science/hal-05347526v2
AB  - Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method that eliminates piezoelectric displacement and synchronization by continuously translating the sample with a motorized stage while recording images on the fly. Depth-resolved information is retrieved via Fourier analysis of the temporal signal at each pixel. This approach enables volumetric imaging over several hundred micrometers within tens of seconds and provides a higher contrast-to-noise ratio than traditional four-phase FF-OCT. Continuous-scanning FF-OCT thus represents a simpler and faster alternative for 3D bio-imaging of living tissues and organoids.
SN  - 2156-7085
SN  - 2156-7085
ER  -