Electricity Free LAMP: A Protocol for Loop-Mediated Isothermal Amplification Using an Exothermic Chemical Reaction and Lyophilized Reagents
- 1University of Texas at Austin
Abstract
Loop-mediated isothermal amplification (LAMP) offers a promising alternative to PCR for low-resource settings, but practical barriers remain. For example there is often the need for reliable electricity, precision heating equipment, and cold-chain storage of reagents. Here, we describe a rudimentary but functional platform that integrates (i) a non-electric, chemically driven water bath capable of sustaining LAMP-relevant temperatures and (ii) lyophilized LAMP assays compatible with simple sample inputs such as diluted saliva or oral rinse. We demonstrate successful LAMP amplification under multiple heating configurations, including a thermos-based chemical bath and field-mimicking setups. This work provides proof-of-concept for a low-cost, portable molecular diagnostic workflow that minimizes infrastructure requirements while maintaining analytical performance.
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BibTeX
@misc{grandhe2026electricity,
title = {{Electricity Free LAMP: A Protocol for Loop-Mediated Isothermal Amplification Using an Exothermic Chemical Reaction and Lyophilized Reagents}},
author = {K. K. Grandhe and T. E. Riedel},
howpublished = {medRxiv},
year = {2026},
month = nov,
doi = {10.64898/2026.11.06.26365637},
url = {https://www.medrxiv.org/content/10.64898/2026.11.06.26365637v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - Electricity Free LAMP: A Protocol for Loop-Mediated Isothermal Amplification Using an Exothermic Chemical Reaction and Lyophilized Reagents
AU - K. K. Grandhe
AU - T. E. Riedel
PY - 2026
DA - 2026/11/06
PB - medRxiv
DO - 10.64898/2026.11.06.26365637
UR - https://www.medrxiv.org/content/10.64898/2026.11.06.26365637v1
AB - Loop-mediated isothermal amplification (LAMP) offers a promising alternative to PCR for low-resource settings, but practical barriers remain. For example there is often the need for reliable electricity, precision heating equipment, and cold-chain storage of reagents. Here, we describe a rudimentary but functional platform that integrates (i) a non-electric, chemically driven water bath capable of sustaining LAMP-relevant temperatures and (ii) lyophilized LAMP assays compatible with simple sample inputs such as diluted saliva or oral rinse. We demonstrate successful LAMP amplification under multiple heating configurations, including a thermos-based chemical bath and field-mimicking setups. This work provides proof-of-concept for a low-cost, portable molecular diagnostic workflow that minimizes infrastructure requirements while maintaining analytical performance.
ER -