Bioactive Composition and In Vitro Antioxidant Properties of Wild Blackberry (Rubus spp.) and Blueberry (Vaccinium spp.) Populations
- 1Department of Chemistry and Chemical Processing Technologies, Biochemistry Program, Maçka Vocational School, Karadeniz Technical University, Maçka, 61750 Trabzon, Türkiye
- 2Bee and Bee Products Application and Research Center (Apimer), Karadeniz Technical University, Ortahisar, 61080 Trabzon, Türkiye
Abstract
This study comparatively evaluated the phenolic profiles and antioxidant capacities of wild blackberry (Rubus spp., n = 5) and blueberry (Vaccinium spp., n = 4) fruits naturally grown in the high-altitude Akılbaba Region (Giresun) of the Eastern Black Sea region (Türkiye). Methanolic extracts of fresh fruits were assessed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities using, the ferric reducing antioxidant power (FRAP) and DPPH assays, while individual phenolic compositions were quantified against 25 standards using RP-HPLC-PDA. The mean TPC and TFC values were determined as 4.49 ± 0.29 mg GAE/g FW and 0.06 ± 0.03 mg QE/g FW for blackberry, and 5.86 ± 1.25 mg GAE/g FW and 0.47 ± 0.13 mg QE/g FW for blueberry, respectively. Regarding antioxidant activity, FRAP values were determined as 96.08 ± 9.92 µmol FeSO4∙7H2O E/g FW for blackberry and 95.30 ± 13.26 µmol FeSO4∙7H2O E/g FW for blueberry samples. In the chromatographic analysis, blackberry was primarily distinguished by substantial concentrations of ellagic and gallic acids, while blueberry was characterized by chlorogenic acid as the major phenolic constituent. In contrast, chrysin and pinocembrin were detected as common flavonoid markers in all samples. Overall, although these two wild berry groups exhibited marked differences in their individual phenolic profiles, their antioxidant responses were assay-dependent, with comparable FRAP values but significantly stronger DPPH radical-scavenging activity in blackberry.
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BibTeX
@article{karahalil2026bioactive,
title = {{Bioactive Composition and In Vitro Antioxidant Properties of Wild Blackberry (Rubus spp.) and Blueberry (Vaccinium spp.) Populations}},
author = {Fatma Yaylacı Karahalil},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31213811},
url = {https://www.mdpi.com/1420-3049/31/21/3811},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Bioactive Composition and In Vitro Antioxidant Properties of Wild Blackberry (Rubus spp.) and Blueberry (Vaccinium spp.) Populations
AU - Fatma Yaylacı Karahalil
PY - 2026
DA - 2026/10/22
JO - Molecules
DO - 10.3390/molecules31213811
UR - https://www.mdpi.com/1420-3049/31/21/3811
AB - This study comparatively evaluated the phenolic profiles and antioxidant capacities of wild blackberry (Rubus spp., n = 5) and blueberry (Vaccinium spp., n = 4) fruits naturally grown in the high-altitude Akılbaba Region (Giresun) of the Eastern Black Sea region (Türkiye). Methanolic extracts of fresh fruits were assessed for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities using, the ferric reducing antioxidant power (FRAP) and DPPH assays, while individual phenolic compositions were quantified against 25 standards using RP-HPLC-PDA. The mean TPC and TFC values were determined as 4.49 ± 0.29 mg GAE/g FW and 0.06 ± 0.03 mg QE/g FW for blackberry, and 5.86 ± 1.25 mg GAE/g FW and 0.47 ± 0.13 mg QE/g FW for blueberry, respectively. Regarding antioxidant activity, FRAP values were determined as 96.08 ± 9.92 µmol FeSO4∙7H2O E/g FW for blackberry and 95.30 ± 13.26 µmol FeSO4∙7H2O E/g FW for blueberry samples. In the chromatographic analysis, blackberry was primarily distinguished by substantial concentrations of ellagic and gallic acids, while blueberry was characterized by chlorogenic acid as the major phenolic constituent. In contrast, chrysin and pinocembrin were detected as common flavonoid markers in all samples. Overall, although these two wild berry groups exhibited marked differences in their individual phenolic profiles, their antioxidant responses were assay-dependent, with comparable FRAP values but significantly stronger DPPH radical-scavenging activity in blackberry.
SN - 1420-3049
ER -