Platelet-released growth factors promote pro-angiogenic effects in endothelial cells: a translational perspective on wound healing and regenerative medicine
Abstract
Platelet-Released Growth Factors (PRGF) are a standardized, cell- and fibrin-free platelet lysate with translational potential for tissue repair. PRGF enhance tissue regeneration, particularly in contexts where angiogenesis is critical for restoring perfusion in chronic and non-healing wounds. Here, we present the first in vitro study demonstrating that PRGF exert pro-angiogenic effects at a working concentration of 10%. The study employs an endothelial cell-based model integrating targeted gene expression analysis, array-based profiling of angiogenesis-related proteins, and functional endothelial tube formation assays. In this setting, screening-level transcriptional and proteomic profiling indicated higher transcript levels of angiogenesis-related genes and increased signals of representative angiogenesis-associated mediators (e.g., Platelet Factor 4, Angiopoietin-1, Angiogenin) following PRGF stimulation. Functional tube formation assays demonstrated significant enhancement of multiple pro-angiogenic parameters in response to PRGF, including number of pieces (P < 0.001), junctions (P < 0.01), and segments (P < 0.05). These findings suggest that 10% PRGF may support angiogenesis and tissue regeneration and provide a foundation for further validation in more complex multicellular systems and in vivo models.
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BibTeX
@article{singh2026plateletreleased,
title = {{Platelet-released growth factors promote pro-angiogenic effects in endothelial cells: a translational perspective on wound healing and regenerative medicine}},
author = {Michael Singh and Karina Zitta and Thilo Wedel and Ralph Lucius and François Cossais and Peter Behrendt and Sabine Fuchs and Jan-Tobias Weitkamp and Rouven Berndt and Olga Zimmermann and Tim Schricker and Andreas Bayer and Martin Albrecht},
journal = {Current Stem Cell Research \& Therapy},
year = {2026},
month = aug,
doi = {10.1186/s13287-026-05214-y},
url = {https://normandie-univ.hal.science/hal-05771356v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Platelet-released growth factors promote pro-angiogenic effects in endothelial cells: a translational perspective on wound healing and regenerative medicine
AU - Michael Singh
AU - Karina Zitta
AU - Thilo Wedel
AU - Ralph Lucius
AU - François Cossais
AU - Peter Behrendt
AU - Sabine Fuchs
AU - Jan-Tobias Weitkamp
AU - Rouven Berndt
AU - Olga Zimmermann
AU - Tim Schricker
AU - Andreas Bayer
AU - Martin Albrecht
PY - 2026
DA - 2026/08/05
JO - Current Stem Cell Research & Therapy
DO - 10.1186/s13287-026-05214-y
UR - https://normandie-univ.hal.science/hal-05771356v1
AB - Platelet-Released Growth Factors (PRGF) are a standardized, cell- and fibrin-free platelet lysate with translational potential for tissue repair. PRGF enhance tissue regeneration, particularly in contexts where angiogenesis is critical for restoring perfusion in chronic and non-healing wounds. Here, we present the first in vitro study demonstrating that PRGF exert pro-angiogenic effects at a working concentration of 10%. The study employs an endothelial cell-based model integrating targeted gene expression analysis, array-based profiling of angiogenesis-related proteins, and functional endothelial tube formation assays. In this setting, screening-level transcriptional and proteomic profiling indicated higher transcript levels of angiogenesis-related genes and increased signals of representative angiogenesis-associated mediators (e.g., Platelet Factor 4, Angiopoietin-1, Angiogenin) following PRGF stimulation. Functional tube formation assays demonstrated significant enhancement of multiple pro-angiogenic parameters in response to PRGF, including number of pieces (P < 0.001), junctions (P < 0.01), and segments (P < 0.05). These findings suggest that 10% PRGF may support angiogenesis and tissue regeneration and provide a foundation for further validation in more complex multicellular systems and in vivo models.
SN - 1574-888X
SN - 2212-3946
ER -