A pyoverdine-type metallophore is required for lanthanide-dependent growth in Pseudomonas putida
- 1National Laboratory of the Rockies
Abstract
Lanthanides (Ln) are recently discovered cofactors for alcohol metabolism in a growing number of bacteria, including the soil-dwelling bacterium Pseudomonas putida, yet the mechanisms for Ln uptake are poorly defined. Here, we discovered that the non-ribosomal peptide synthase biosynthetic gene cluster, pvdLIJD, was essential to Ln-dependent growth in P. putida. Pyoverdine G4R and its precursor ferribactin were identified as pvdLIJD gene products with La-responsive abundances, and both chelated La as well as iron. Deletion of the putative pyoverdine outer membrane transport system encoded by fpvA and exbBD:tonB were strongly linked to Ln-dependent growth in a concentration-dependent manner. Global transcriptomes were remodeled in response to La, but weak evidence of transcriptional pvdLIJD regulation and no evidence of discernment between La provided as chloride versus oxide were observed. Overall, this work establishes pyoverdines as metallophores involved in La uptake, re-framing them from strictly iron-scavenging siderophores to dual-purpose metallophores with Ln-binding activity.
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BibTeX
@misc{rasmussen2026pyoverdinetype,
title = {{A pyoverdine-type metallophore is required for lanthanide-dependent growth in Pseudomonas putida}},
author = {K. Rasmussen and J. Michael and A. F. Benson and K. J. Ramirez and M. A. Ingraham and A. T. Aron and A. Z. Werner},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.08.750064},
url = {https://www.biorxiv.org/content/10.64898/2026.09.08.750064v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - A pyoverdine-type metallophore is required for lanthanide-dependent growth in Pseudomonas putida
AU - K. Rasmussen
AU - J. Michael
AU - A. F. Benson
AU - K. J. Ramirez
AU - M. A. Ingraham
AU - A. T. Aron
AU - A. Z. Werner
PY - 2026
DA - 2026/09/08
PB - bioRxiv
DO - 10.64898/2026.09.08.750064
UR - https://www.biorxiv.org/content/10.64898/2026.09.08.750064v1
AB - Lanthanides (Ln) are recently discovered cofactors for alcohol metabolism in a growing number of bacteria, including the soil-dwelling bacterium Pseudomonas putida, yet the mechanisms for Ln uptake are poorly defined. Here, we discovered that the non-ribosomal peptide synthase biosynthetic gene cluster, pvdLIJD, was essential to Ln-dependent growth in P. putida. Pyoverdine G4R and its precursor ferribactin were identified as pvdLIJD gene products with La-responsive abundances, and both chelated La as well as iron. Deletion of the putative pyoverdine outer membrane transport system encoded by fpvA and exbBD:tonB were strongly linked to Ln-dependent growth in a concentration-dependent manner. Global transcriptomes were remodeled in response to La, but weak evidence of transcriptional pvdLIJD regulation and no evidence of discernment between La provided as chloride versus oxide were observed. Overall, this work establishes pyoverdines as metallophores involved in La uptake, re-framing them from strictly iron-scavenging siderophores to dual-purpose metallophores with Ln-binding activity.
ER -