Skip to content
DraconiticResearch
Search Sources API
201,818 studies31 sourcesUpdated 22 min ago
Peer-reviewedCC BY-NC-ND 4.0Poultry Science

circMAPKBP1 functions as a miR-143-y sponge to regulate quail myoblast proliferation and differentiation

  • Jing Liu1,2
  • Hongxia Jiang1,2
  • Xuwen Huang1,2
  • Yuxiang Wang1,2
  • Guiyan Wang3
  • Xiaolong Hu1,2
  • Huirong Mao1,2
  • Sanfeng Liu1,2
  • Qingjie Zeng1
  • Biao Chen1,2
  1. 1College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, China
  2. 2Poultry Institute, Jiangxi Agricultural University, Nanchang, 330045, China
  3. 3Yichun Academy of Sciences, Yichun, Jiangxi, 336000, China
Read full textDOICite

Abstract

The quail (Coturnix japonica) is an economically important poultry species and a useful avian model for muscle biology. Although circular RNA (circRNA)–miRNA competitive endogenous RNA (ceRNA) networks have been described in chicken and duck skeletal muscle, their roles in quail remain poorly defined. We identified circMAPKBP1, generated from the mitogen-activated protein kinase binding protein 1 (MAPKBP1), as highly expressed in quail skeletal muscle and myoblasts. Gain- and loss-of-function experiments in quail myoblast 7 (QM7) cells showed that circMAPKBP1 promotes proliferation while inhibiting differentiation. Dual-luciferase reporter assays demonstrated direct binding between circMAPKBP1 and miR-143-y. Overexpression of miR-143-y promoted both proliferation and differentiation, and its expression pattern was reciprocal to that of circMAPKBP1 during differentiation. RNA-seq of miR-143-y-overexpressing cells revealed significant enrichment of downregulated genes in the mitogen-activated protein kinase (MAPK) signaling pathway. These results establish, for the first time, that the circMAPKBP1/miR-143-y axis modulates quail myogenesis, with the sponge mechanism most clearly explaining the opposing effects on differentiation. The findings provide molecular targets and a mechanistic framework for genetic improvement of muscle traits in poultry.

Related

  1. Dynamic increases in p53 expression suppress human myoblast differentiation
    Ingrid R. Aragon, Neha Patole, Ajay Vachanaram +7
    Frontiers in cell and developmental biologyPeer-reviewedCC BYFull textGeneral biochemistryEurope PMC
  2. mtDNA alterations in muscle progenitors determine myoblast differentiation and disrupt skeletal muscle architecture
    Ayesha Sen, Olivier Baris, Yulia Schaumkessel +5
    Cell ReportsPeer-reviewedCC BYMolecular biology & gene regulationHAL
  3. Tceal7 is a BRG1-regulated target of calcineurin signaling that promotes myoblast differentiation
    Sandhya Yadav, Tapan Sharma, Monserrat Olea-Flores +2
    PLOS OnePeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationDevelopmental biologyPLOS
  4. ALIX-dependent small extracellular vesicles from tissue-resident mesenchymal cells regulate pancreatic β cell proliferation in obesity
    Yuhei Uehara, Hirofumi Nagao, Aoki Tobimatsu +9
    Science AdvancesPeer-reviewedCC BY-NCMolecular biology & gene regulationCrossref
  5. SMURFs regulate mesendoderm differentiation through SMAD2-dependent ubiquitination of CDK9
    Jee-Hye Shin, Xuan Xu, Tiaojiang Xiao +7
    Cell ReportsPeer-reviewedCC BY-NC-ND 4.0Molecular biology & gene regulationCrossref

Cite

BibTeXRIS
BibTeX
@article{liu2026circmapkbp1,
  title = {{circMAPKBP1 functions as a miR-143-y sponge to regulate quail myoblast proliferation and differentiation}},
  author = {Jing Liu and Hongxia Jiang and Xuwen Huang and Yuxiang Wang and Guiyan Wang and Xiaolong Hu and Huirong Mao and Sanfeng Liu and Qingjie Zeng and Biao Chen},
  journal = {Poultry Science},
  year = {2026},
  month = oct,
  doi = {10.1016/j.psj.2026.107982},
  url = {https://doi.org/10.1016/j.psj.2026.107982},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - circMAPKBP1 functions as a miR-143-y sponge to regulate quail myoblast proliferation and differentiation
AU  - Jing Liu
AU  - Hongxia Jiang
AU  - Xuwen Huang
AU  - Yuxiang Wang
AU  - Guiyan Wang
AU  - Xiaolong Hu
AU  - Huirong Mao
AU  - Sanfeng Liu
AU  - Qingjie Zeng
AU  - Biao Chen
PY  - 2026
DA  - 2026/10/17
JO  - Poultry Science
DO  - 10.1016/j.psj.2026.107982
UR  - https://doi.org/10.1016/j.psj.2026.107982
AB  - The quail (Coturnix japonica) is an economically important poultry species and a useful avian model for muscle biology. Although circular RNA (circRNA)–miRNA competitive endogenous RNA (ceRNA) networks have been described in chicken and duck skeletal muscle, their roles in quail remain poorly defined. We identified circMAPKBP1, generated from the mitogen-activated protein kinase binding protein 1 (MAPKBP1), as highly expressed in quail skeletal muscle and myoblasts. Gain- and loss-of-function experiments in quail myoblast 7 (QM7) cells showed that circMAPKBP1 promotes proliferation while inhibiting differentiation. Dual-luciferase reporter assays demonstrated direct binding between circMAPKBP1 and miR-143-y. Overexpression of miR-143-y promoted both proliferation and differentiation, and its expression pattern was reciprocal to that of circMAPKBP1 during differentiation. RNA-seq of miR-143-y-overexpressing cells revealed significant enrichment of downregulated genes in the mitogen-activated protein kinase (MAPK) signaling pathway. These results establish, for the first time, that the circMAPKBP1/miR-143-y axis modulates quail myogenesis, with the sponge mechanism most clearly explaining the opposing effects on differentiation. The findings provide molecular targets and a mechanistic framework for genetic improvement of muscle traits in poultry.
SN  - 0032-5791
SN  - 1525-3171
ER  -