Factors associated with microbiological non-compliance in ready-to-eat foods: A seven-year analysis of official food safety surveillance data in a tropical low- and middle-income country
- 1GRIVI Research Group, Faculty of Medicine, Universidad Cooperativa de Colombia, Villavicencio, Colombia
- 2GISSME Research Group, Secretaría de Salud del Meta, Villavicencio, Colombia
- 3GEDOVAZ Research Group, Faculty of Business Administration, Universidad Cooperativa de Colombia, Villavicencio, Colombia
Abstract
Food safety is an important component of global public health, particularly in low- and middle-income countries (LMICs). This study aimed to identify factors associated with microbiological non-compliance in ready-to-eat (RTE) foods using seven years of official surveillance data from the Colombian Orinoquía region. RTE foods were defined as foods intended for immediate consumption without additional cooking or thermal processing. A retrospective analysis was conducted on 1,868 samples collected between 2017 and 2023, of which 1,846 were included in inferential analyses. Microbiological non-compliance, defined as failure to meet at least one regulatory microbiological criterion, was the primary outcome. Associations were assessed using multivariable logistic regression, complemented by descriptive and exploratory analyses, including heatmaps and principal component analysis. Microbiological non-compliance was identified in 39.5% of the evaluated samples. Hygiene indicators predominated, particularly total coliforms (29.0%), aerobic mesophilic bacteria (12.7%), and Escherichia coli (12.3%), whereas foodborne pathogens were infrequently detected. Raw or highly handled foods had higher odds of microbiological non-compliance than thermally processed foods (OR = 8.40; 95% CI: 6.59–10.72). Higher odds were also observed for samples from rural or dispersed municipalities (OR = 1.31; 95% CI: 1.01–1.70) and samples collected in 2020 (OR = 1.78; 95% CI: 1.16–2.74). Exploratory analyses revealed heterogeneous microbiological profiles across temporal, territorial, operational, and food-related dimensions, with a consistent predominance of hygiene indicators. Overall, microbiological non-compliance was mainly associated with food matrix type, territorial characteristics, and sampling year. The predominance of hygiene indicators supports incorporating hygienic quality and process control alongside pathogen detection into microbiological surveillance. These findings provide evidence to support risk-based food safety surveillance and targeted prioritization strategies in tropical low- and middle-income settings.
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BibTeX
@article{rodriguez2026factors,
title = {{Factors associated with microbiological non-compliance in ready-to-eat foods: A seven-year analysis of official food safety surveillance data in a tropical low- and middle-income country}},
author = {L. Marcela Montilla Rodríguez and Jorge Alejandro Obando Bastidas and Hugo Andrés Rodríguez Fajardo and Astrid Magaly Mora López and Shirley Viviana Tique Aragón and Dora Patricia Rozo Cruz and Edgar Calderón Vega and Norma Cristina Pavas Escobar and Laura Nathalia Obando Vargas and Jean Paul Farfán and Marina Sthella Gonzalez Robayo},
journal = {PLOS Global Public Health},
year = {2026},
month = oct,
doi = {10.1371/journal.pgph.0006492},
url = {https://journals.plos.org/globalpublichealth/article?id=10.1371/journal.pgph.0006492},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Factors associated with microbiological non-compliance in ready-to-eat foods: A seven-year analysis of official food safety surveillance data in a tropical low- and middle-income country
AU - L. Marcela Montilla Rodríguez
AU - Jorge Alejandro Obando Bastidas
AU - Hugo Andrés Rodríguez Fajardo
AU - Astrid Magaly Mora López
AU - Shirley Viviana Tique Aragón
AU - Dora Patricia Rozo Cruz
AU - Edgar Calderón Vega
AU - Norma Cristina Pavas Escobar
AU - Laura Nathalia Obando Vargas
AU - Jean Paul Farfán
AU - Marina Sthella Gonzalez Robayo
PY - 2026
DA - 2026/10/05
JO - PLOS Global Public Health
DO - 10.1371/journal.pgph.0006492
UR - https://journals.plos.org/globalpublichealth/article?id=10.1371/journal.pgph.0006492
AB - Food safety is an important component of global public health, particularly in low- and middle-income countries (LMICs). This study aimed to identify factors associated with microbiological non-compliance in ready-to-eat (RTE) foods using seven years of official surveillance data from the Colombian Orinoquía region. RTE foods were defined as foods intended for immediate consumption without additional cooking or thermal processing. A retrospective analysis was conducted on 1,868 samples collected between 2017 and 2023, of which 1,846 were included in inferential analyses. Microbiological non-compliance, defined as failure to meet at least one regulatory microbiological criterion, was the primary outcome. Associations were assessed using multivariable logistic regression, complemented by descriptive and exploratory analyses, including heatmaps and principal component analysis. Microbiological non-compliance was identified in 39.5% of the evaluated samples. Hygiene indicators predominated, particularly total coliforms (29.0%), aerobic mesophilic bacteria (12.7%), and Escherichia coli (12.3%), whereas foodborne pathogens were infrequently detected. Raw or highly handled foods had higher odds of microbiological non-compliance than thermally processed foods (OR = 8.40; 95% CI: 6.59–10.72). Higher odds were also observed for samples from rural or dispersed municipalities (OR = 1.31; 95% CI: 1.01–1.70) and samples collected in 2020 (OR = 1.78; 95% CI: 1.16–2.74). Exploratory analyses revealed heterogeneous microbiological profiles across temporal, territorial, operational, and food-related dimensions, with a consistent predominance of hygiene indicators. Overall, microbiological non-compliance was mainly associated with food matrix type, territorial characteristics, and sampling year. The predominance of hygiene indicators supports incorporating hygienic quality and process control alongside pathogen detection into microbiological surveillance. These findings provide evidence to support risk-based food safety surveillance and targeted prioritization strategies in tropical low- and middle-income settings.
SN - 2767-3375
ER -