Fecal Metabolic and Microbial Responses to Five Acute Fueling Strategies During a 60-Minute Rowing Protocol: A Randomized Crossover Multi-Omics Pilot Study.
- 1Department of Physical Education, Capital Normal University, Beijing 100048, China.
Abstract
Background/objectives
We compared next-morning fecal metabolic and microbial responses to five acute fueling strategies.Methods
Eight male recreational rowers with complete specimens from 12 crossover completers received an erythritol comparator, two carbohydrate doses, fixed carbohydrate-protein, and personalized carbohydrate-protein (P-CPS). Eighty specimens yielded 40 paired changes. Participant-blocked, period-adjusted models used restricted permutations and Benjamini-Hochberg false discovery rate (FDR) correction.Results
No primary global test passed across-modality FDR correction: SCFAs, p = 0.0321, q = 0.0963; metabolome, q = 0.1334; microbiome, q = 0.2813. No metabolite or originally annotated genus passed its omnibus FDR family. Post hoc SILVA 138.2 reclassification retained a nonsignificant global result but identified three low-count genus-level omnibus signals. Secondary change-score contrasts indicated higher isovaleric and isobutyric post/pre fold changes under P-CPS, fixed carbohydrate-protein, and high carbohydrate than under the comparator. However, no concentration or composite-endpoint contrast remained FDR-positive in post hoc models adjusting for same-trial pre-trial values. Caproic acid evidence also weakened with expanded correction and analytical-range checks. Two metabolome-SCFA principal-component correlations survived FDR correction and persisted after exclusion of overlapping annotations.Conclusions
This pilot identified model-sensitive secondary chemical signals without FDR-supported global intervention effects. Baseline imbalance, unmeasured beverage osmolality and fecal water, and quantification limitations preclude attributing these signals to increased fermentation or nutritional benefit.
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BibTeX
@article{wang2026fecal,
title = {{Fecal Metabolic and Microbial Responses to Five Acute Fueling Strategies During a 60-Minute Rowing Protocol: A Randomized Crossover Multi-Omics Pilot Study.}},
author = {Xiangyu Wang and Jian Huang},
journal = {Metabolites},
year = {2026},
month = sep,
doi = {10.3390/metabo16100718},
url = {https://europepmc.org/article/MED/42941194},
note = {CC BY},
}
RIS
TY - JOUR
TI - Fecal Metabolic and Microbial Responses to Five Acute Fueling Strategies During a 60-Minute Rowing Protocol: A Randomized Crossover Multi-Omics Pilot Study.
AU - Xiangyu Wang
AU - Jian Huang
PY - 2026
DA - 2026/09/28
JO - Metabolites
DO - 10.3390/metabo16100718
UR - https://europepmc.org/article/MED/42941194
AB - Background/objectives We compared next-morning fecal metabolic and microbial responses to five acute fueling strategies.Methods Eight male recreational rowers with complete specimens from 12 crossover completers received an erythritol comparator, two carbohydrate doses, fixed carbohydrate-protein, and personalized carbohydrate-protein (P-CPS). Eighty specimens yielded 40 paired changes. Participant-blocked, period-adjusted models used restricted permutations and Benjamini-Hochberg false discovery rate (FDR) correction.Results No primary global test passed across-modality FDR correction: SCFAs, p = 0.0321, q = 0.0963; metabolome, q = 0.1334; microbiome, q = 0.2813. No metabolite or originally annotated genus passed its omnibus FDR family. Post hoc SILVA 138.2 reclassification retained a nonsignificant global result but identified three low-count genus-level omnibus signals. Secondary change-score contrasts indicated higher isovaleric and isobutyric post/pre fold changes under P-CPS, fixed carbohydrate-protein, and high carbohydrate than under the comparator. However, no concentration or composite-endpoint contrast remained FDR-positive in post hoc models adjusting for same-trial pre-trial values. Caproic acid evidence also weakened with expanded correction and analytical-range checks. Two metabolome-SCFA principal-component correlations survived FDR correction and persisted after exclusion of overlapping annotations.Conclusions This pilot identified model-sensitive secondary chemical signals without FDR-supported global intervention effects. Baseline imbalance, unmeasured beverage osmolality and fecal water, and quantification limitations preclude attributing these signals to increased fermentation or nutritional benefit.
SN - 2218-1989
ER -