Multienzyme cascade biotransformation of L-Tyrosine to Danshensu by engineered Escherichia coli
- 1Henan Key Laboratory of Tea Plant Biology, College of Tea and Food Science, Xinyang Normal University, Xinyang, Henan, China.
- 2Dabie Mountain Laboratory, Xinyang, Henan, China.
- 3Henan International Joint Laboratory of Tea-oil tree Biology and High-Value Utilization, College of Tea and Food Science, Xinyang Normal University, Xinyang, Henan, China.
Abstract
Danshensu (DSS), a water-soluble bioactive compound with significant antioxidant, anti-inflammatory, cardioprotective and neuroprotective properties, is widely utilized in cardiovascular and cerebrovascular disease. In this study, we established a multi-enzyme cascade system in engineered Escherichia coli to synthesize DSS from L-Tyrosine. The initial co-expression of 4-hydroxyphenylacetate 3-monooxygenase (HpaBC), L-amino acid deaminase (aadL), glucose dehydrogenase (gdh) and D-lactate dehydrogenase (ldhD) yielded 5.1 mM DSS. The production of DSS increased to 6.53 mM through optimizing plasmid copy number. After optimizing the conditions, the yield of DSS reached 8.66 mM from strain 6. Finally, whole cell biotransformation using strain 6 in bioreactor yielded 19.47 mM DSS within 32h. These findings provide valuable insights for the scalable biosynthesis of pharmaceutically important compound of DSS from naturally available substrates of L-Tyrosine.
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BibTeX
@article{xiong2026multienzyme,
title = {{Multienzyme cascade biotransformation of L-Tyrosine to Danshensu by engineered Escherichia coli}},
author = {Tianzhen Xiong and Shuqing Li and Xiaoya Li and Xinmeng Li and Mengdan Wu and Jinru Liu and Jinxiu Guo and Xinrui Li},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0361484},
url = {https://doi.org/10.1371/journal.pone.0361484},
note = {CC BY},
}
RIS
TY - JOUR
TI - Multienzyme cascade biotransformation of L-Tyrosine to Danshensu by engineered Escherichia coli
AU - Tianzhen Xiong
AU - Shuqing Li
AU - Xiaoya Li
AU - Xinmeng Li
AU - Mengdan Wu
AU - Jinru Liu
AU - Jinxiu Guo
AU - Xinrui Li
PY - 2026
DA - 2026/10/23
JO - PLOS One
DO - 10.1371/journal.pone.0361484
UR - https://doi.org/10.1371/journal.pone.0361484
AB - Danshensu (DSS), a water-soluble bioactive compound with significant antioxidant, anti-inflammatory, cardioprotective and neuroprotective properties, is widely utilized in cardiovascular and cerebrovascular disease. In this study, we established a multi-enzyme cascade system in engineered Escherichia coli to synthesize DSS from L-Tyrosine. The initial co-expression of 4-hydroxyphenylacetate 3-monooxygenase (HpaBC), L-amino acid deaminase (aadL), glucose dehydrogenase (gdh) and D-lactate dehydrogenase (ldhD) yielded 5.1 mM DSS. The production of DSS increased to 6.53 mM through optimizing plasmid copy number. After optimizing the conditions, the yield of DSS reached 8.66 mM from strain 6. Finally, whole cell biotransformation using strain 6 in bioreactor yielded 19.47 mM DSS within 32h. These findings provide valuable insights for the scalable biosynthesis of pharmaceutically important compound of DSS from naturally available substrates of L-Tyrosine.
SN - 1932-6203
ER -