Laser-Induced Graphene Paper-Based Microfluidic Platform for Dual-Mode Electrochemical and Colorimetric Detection of Glucose
- 1Academia Sinica , , Nankang , ,
Abstract
Microfluidic paper-based analytical devices (μPADs) provide an attractive platform for diagnostics owing to their low cost, passive fluid transport, and ease of fabrication. However, integrating dual-sensing capabilities into a single paper device while maintaining a simple manufacturing process remains challenging. Here, we present a foldable dual-mode microfluidic paper-based analytical device (DM-μPAD) that integrates laser-induced graphene (LIG)-based electrochemical sensing with enzyme-mediated colorimetric detection. A straightforward laser-patterning strategy was used to fabricate LIG electrodes directly patterned onto the polyimide tape-covered cellulose paper via a one-step laser engraving process, forming a conductive graphene network that serves as an electrochemical sensing module. A screen-printing process was used to form hydrophobic perfluoropolyether barriers, which define capillary-driven microfluidic channels that guide sample transport between sensing regions. The folded architecture enhances fluid-electrode interaction and enables simultaneous electrochemical and colorimetric measurements within a single analytical platform without electrode transfer or complex assembly. This dual-readout technique provides cross-validation of results to enhance detection reliability. The proposed DM-μPAD demonstrated good selectivity against common nonspecific compounds, a wide linear detection range of 0.01 to 3.0 mM, and a low detection limit of 9.23 μM in the electrochemical mode, while displaying a dynamic detection range of 0.1 to 2.0 mM with a detection limit of 57.9 μM in the colorimetric mode. Furthermore, the platform maintained high reproducibility, mechanical flexibility, and environmental stability. This work highlights the potential of laser-induced graphene-based paper microfluidics as a versatile platform for developing portable, cost-effective, and multiplexed biosensing devices.
Related
- Forensic electrochemistry on a drop: bromocresol green-modified screen-printed platforms for dual colorimetric-electrochemical detection of scopolamine.
- Synergistic PVA/zein electrospun nanofiber array with curcumin-alizarin: a water-resistant, sensitive colorimetric platform for graded pork freshness monitoring.
- Municipal wastewater sludge/laser-induced graphene nanozyme for machine-learning-assisted smartphone colorimetric tetracycline sensing
- Surface modification strategies for carbon nanotubes and graphene materials: advances in electrochemical catalysis
- Recapitulating In Vivo Pharmacokinetics and Size‐Dependent Nanomedicine Delivery in a Microfluidic Platform
BibTeX
@article{yeh2026laserinduced,
title = {{Laser-Induced Graphene Paper-Based Microfluidic Platform for Dual-Mode Electrochemical and Colorimetric Detection of Glucose}},
author = {Che-Liang Yeh and Syed Atif Ali and Wen-Ti Wu and Hsiung-Lin Tu},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c09156},
url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c09156/5457744/Laser-Induced-Graphene-Paper-Based-Microfluidic},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Laser-Induced Graphene Paper-Based Microfluidic Platform for Dual-Mode Electrochemical and Colorimetric Detection of Glucose
AU - Che-Liang Yeh
AU - Syed Atif Ali
AU - Wen-Ti Wu
AU - Hsiung-Lin Tu
PY - 2026
DA - 2026/10/24
JO - ACS Omega
DO - 10.1021/acsomega.6c09156
UR - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c09156/5457744/Laser-Induced-Graphene-Paper-Based-Microfluidic
AB - Microfluidic paper-based analytical devices (μPADs) provide an attractive platform for diagnostics owing to their low cost, passive fluid transport, and ease of fabrication. However, integrating dual-sensing capabilities into a single paper device while maintaining a simple manufacturing process remains challenging. Here, we present a foldable dual-mode microfluidic paper-based analytical device (DM-μPAD) that integrates laser-induced graphene (LIG)-based electrochemical sensing with enzyme-mediated colorimetric detection. A straightforward laser-patterning strategy was used to fabricate LIG electrodes directly patterned onto the polyimide tape-covered cellulose paper via a one-step laser engraving process, forming a conductive graphene network that serves as an electrochemical sensing module. A screen-printing process was used to form hydrophobic perfluoropolyether barriers, which define capillary-driven microfluidic channels that guide sample transport between sensing regions. The folded architecture enhances fluid-electrode interaction and enables simultaneous electrochemical and colorimetric measurements within a single analytical platform without electrode transfer or complex assembly. This dual-readout technique provides cross-validation of results to enhance detection reliability. The proposed DM-μPAD demonstrated good selectivity against common nonspecific compounds, a wide linear detection range of 0.01 to 3.0 mM, and a low detection limit of 9.23 μM in the electrochemical mode, while displaying a dynamic detection range of 0.1 to 2.0 mM with a detection limit of 57.9 μM in the colorimetric mode. Furthermore, the platform maintained high reproducibility, mechanical flexibility, and environmental stability. This work highlights the potential of laser-induced graphene-based paper microfluidics as a versatile platform for developing portable, cost-effective, and multiplexed biosensing devices.
SN - 2470-1343
ER -