Chromosomal-Level Whole Genome Assemblies of Crassostrea Virginica
- 1Yale University
Abstract
The eastern oyster, Crassostrea virginica, is an ecologically important foundation species and a valuable aquaculture organism. To support genomic studies and future breeding applications, we generated PacBio HiFi, Illumina, and Hi-C sequencing data from wild adult oysters, collected from Long Island Sound and assembled a chromosome-level reference genome. The final assembly spans 529.0 Mb and consists of 10 chromosomes with a scaffold N50 of 55.46 Mb and a contig N50 of 16.0 Mb. The resulting reference genome achieved 99.7% genome completeness and reduced duplicated universal single-copy ortholog content from 18.7% to 1.1%. Genome annotation identified 33,259 protein-coding genes and revealed that 43.2% of the genome is composed of repetitive elements. All sequencing data underwent stringent quality control, and the assembly results were validated through multiple technical evaluations. These dataset and resources of oyster genomics provided future studies such as effective breeding and sustainable farming.
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BibTeX
@misc{cawood2026chromosomallevel,
title = {{Chromosomal-Level Whole Genome Assemblies of Crassostrea Virginica}},
author = {L. Cawood and S. Wang and E. Kleiman and Z. Chen and I. Kleiman and N. Fair and R. Brownell and I. Tikhonova and Z. Li and J. Hoh},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.13.758928},
url = {https://www.biorxiv.org/content/10.64898/2026.10.13.758928v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Chromosomal-Level Whole Genome Assemblies of Crassostrea Virginica
AU - L. Cawood
AU - S. Wang
AU - E. Kleiman
AU - Z. Chen
AU - I. Kleiman
AU - N. Fair
AU - R. Brownell
AU - I. Tikhonova
AU - Z. Li
AU - J. Hoh
PY - 2026
DA - 2026/10/13
PB - bioRxiv
DO - 10.64898/2026.10.13.758928
UR - https://www.biorxiv.org/content/10.64898/2026.10.13.758928v1
AB - The eastern oyster, Crassostrea virginica, is an ecologically important foundation species and a valuable aquaculture organism. To support genomic studies and future breeding applications, we generated PacBio HiFi, Illumina, and Hi-C sequencing data from wild adult oysters, collected from Long Island Sound and assembled a chromosome-level reference genome. The final assembly spans 529.0 Mb and consists of 10 chromosomes with a scaffold N50 of 55.46 Mb and a contig N50 of 16.0 Mb. The resulting reference genome achieved 99.7% genome completeness and reduced duplicated universal single-copy ortholog content from 18.7% to 1.1%. Genome annotation identified 33,259 protein-coding genes and revealed that 43.2% of the genome is composed of repetitive elements. All sequencing data underwent stringent quality control, and the assembly results were validated through multiple technical evaluations. These dataset and resources of oyster genomics provided future studies such as effective breeding and sustainable farming.
SN - 2692-8205
ER -