Deletion of the Envelope gene attenuates SARS-CoV-2 infection by altered spike localization and increased cell-to-cell transmission
4 affiliations
- 1Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine
- 2Center for Vaccine Research, University of Pittsburgh
- 3Department of Cell Biology, University of Pittsburgh School of Medicine
- 4Department of Structural Biology, University of Pittsburgh School of Medicine
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a highly transmissible acute respiratory infection that can result in severe pneumonia and death. Many details of SARS-CoV-2 infection are not fully understood, including the cell biology and host-virus interactions involved in coronavirus assembly and release, in which the Envelope (E) structural protein is instrumental. Deletion of E in other coronaviruses has been shown previously to either attenuate or abrogate infection. To determine the role of E in SARS-CoV-2 virus production and infectivity, we produced a reporter SARS-CoV-2 with or without the E gene deleted using a bacterial artificial chromosome. Replication of ΔE SARS-CoV-2 was attenuated in Vero E6 cells expressing human ACE2 and TMPRSS2 and in human epithelial cell lines. Electron and immunofluorescence microscopy and virology assays showed that ΔE SARS-CoV-2 increased cell surface expression of Spike (S) glycoprotein, leading to reduced S incorporation into ΔE SARS-CoV-2 particles and promotion of increased cell-to-cell transmission that evades neutralizing antibody inhibition. Trans-complementation of E partially rescued ΔE SARS-CoV-2 S incorporation and restored cell-free transmission. In addition to validating the role of E in retention of S in the ER-Golgi intermediate complex (ERGIC), our results showed that a lack of E led to reorganization of the ERGIC and Golgi during SARS-CoV-2 infection. Improved understanding of E in SARS-CoV-2 replication and host pathogenesis may help the development of novel therapeutics.
IMPORTANCE: Non-Spike coronavirus structural proteins, including Envelope, are conserved, making them potential pan-coronavirus therapeutic targets. Many details about these proteins and their roles in viral replication and host pathogenesis are unknown. In this study, we showed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates without Envelope but is attenuated and impaired in virus particle formation, with less Spike incorporated into virions and more Spike expressed on the cell surface compared to wild-type virus. SARS-CoV-2 lacking Envelope transmitted primarily via cell fusion and evaded neutralizing antibodies. In addition, the absence of Envelope resulted in the reorganization of the cell secretory compartment during SARS-CoV-2 infection. A better understanding of how Envelope influences SARS-CoV-2 replication could guide directed design of novel therapeutics for treatment of COVID-19 patients, as well as the potential for pan-coronavirus protection against future coronavirus outbreaks.
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BibTeX
@article{fischer2026deletion,
title = {{Deletion of the Envelope gene attenuates SARS-CoV-2 infection by altered spike localization and increased cell-to-cell transmission}},
author = {Hannah L. Fischer and Shanmugapriya Shanmugapriya and Christopher Kline and W. Paul Duprex and Kevin R. McCarthy and Simon C. Watkins and James F. Conway and Zandrea Ambrose},
journal = {mBio},
year = {2026},
month = nov,
doi = {10.1128/mbio.01574-26},
url = {https://journals.asm.org/doi/10.1128/mbio.01574-26},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Deletion of the Envelope gene attenuates SARS-CoV-2 infection by altered spike localization and increased cell-to-cell transmission
AU - Hannah L. Fischer
AU - Shanmugapriya Shanmugapriya
AU - Christopher Kline
AU - W. Paul Duprex
AU - Kevin R. McCarthy
AU - Simon C. Watkins
AU - James F. Conway
AU - Zandrea Ambrose
PY - 2026
DA - 2026/11/03
JO - mBio
DO - 10.1128/mbio.01574-26
UR - https://journals.asm.org/doi/10.1128/mbio.01574-26
AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a highly transmissible acute respiratory infection that can result in severe pneumonia and death. Many details of SARS-CoV-2 infection are not fully understood, including the cell biology and host-virus interactions involved in coronavirus assembly and release, in which the Envelope (E) structural protein is instrumental. Deletion of E in other coronaviruses has been shown previously to either attenuate or abrogate infection. To determine the role of E in SARS-CoV-2 virus production and infectivity, we produced a reporter SARS-CoV-2 with or without the E gene deleted using a bacterial artificial chromosome. Replication of ΔE SARS-CoV-2 was attenuated in Vero E6 cells expressing human ACE2 and TMPRSS2 and in human epithelial cell lines. Electron and immunofluorescence microscopy and virology assays showed that ΔE SARS-CoV-2 increased cell surface expression of Spike (S) glycoprotein, leading to reduced S incorporation into ΔE SARS-CoV-2 particles and promotion of increased cell-to-cell transmission that evades neutralizing antibody inhibition. Trans-complementation of E partially rescued ΔE SARS-CoV-2 S incorporation and restored cell-free transmission. In addition to validating the role of E in retention of S in the ER-Golgi intermediate complex (ERGIC), our results showed that a lack of E led to reorganization of the ERGIC and Golgi during SARS-CoV-2 infection. Improved understanding of E in SARS-CoV-2 replication and host pathogenesis may help the development of novel therapeutics. IMPORTANCE: Non-Spike coronavirus structural proteins, including Envelope, are conserved, making them potential pan-coronavirus therapeutic targets. Many details about these proteins and their roles in viral replication and host pathogenesis are unknown. In this study, we showed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates without Envelope but is attenuated and impaired in virus particle formation, with less Spike incorporated into virions and more Spike expressed on the cell surface compared to wild-type virus. SARS-CoV-2 lacking Envelope transmitted primarily via cell fusion and evaded neutralizing antibodies. In addition, the absence of Envelope resulted in the reorganization of the cell secretory compartment during SARS-CoV-2 infection. A better understanding of how Envelope influences SARS-CoV-2 replication could guide directed design of novel therapeutics for treatment of COVID-19 patients, as well as the potential for pan-coronavirus protection against future coronavirus outbreaks.
SN - 2150-7511
ER -