Biomarkers indicate altered outer retinal fluid homeostasis in the absence of Kv2.1/Kv8.2 channels.
- 1UNIVERSITY OF IOWA
Abstract
Purpose: Photoreceptor Kv2.1/Kv8.2 voltage-gated potassium channels carry an outward potassium current, helping to set the resting membrane potential and to shape dim light responses. Because potassium flux in the outer retina influences extracellular osmolarity and fluid distribution, we hypothesized that Kv2.1/Kv8.2 channels also contribute to fluid homeostasis in this region of the retina. Methods: OCT imaging was performed in Kv8.2 heterozygous (Het) and knockout (KO) mice aged ~5 weeks under dark- and light-adapted conditions. Light-dark differences in the distance between the external limiting membrane (ELM) and retinal pigment epithelium (RPE) ({Delta}ELM-RPE) were calculated to quantify light-evoked outer retinal expansion, a biomarker associated with changes in subretinal space (SRS) fluid handling. As a secondary outcome, outer nuclear layer (ONL) thickness was also measured under both lighting conditions. Retinal gene expression was assessed by RNA-seq and droplet digital RT-PCR. Retinal protein expression was determined by western blotting and immunolabeling. Results: {Delta}ELM-RPE was significantly reduced in Kv8.2 KO mice compared with Het controls, indicating altered light-evoked outer retinal dynamics compatible with changes in subretinal space fluid handling. ONL thickness exhibited a small but significant light-dark change that was different between genotypes. Transcriptomic analyses revealed upregulation of osmosensitive genes, including osmolyte transporters and aquaporins. AQP1 protein expression in photoreceptors increased. Conclusions: These findings are consistent with altered outer retinal fluid homeostasis following loss of Kv8.2 and support a model in which photoreceptor potassium efflux contributes to osmotic water movement into the subretinal space.
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BibTeX
@misc{laird2026biomarkers,
title = {{Biomarkers indicate altered outer retinal fluid homeostasis in the absence of Kv2.1/Kv8.2 channels.}},
author = {J. G. Laird and J. Soetedjo and S. M. Inamdar and A. R. Bock and E. S. Ataman and M. A. Pufall and B. A. Berkowitz and S. A. Baker},
howpublished = {bioRxiv},
year = {2026},
month = jun,
doi = {10.64898/2026.06.27.734996},
url = {https://www.biorxiv.org/content/10.64898/2026.06.27.734996v2},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - Biomarkers indicate altered outer retinal fluid homeostasis in the absence of Kv2.1/Kv8.2 channels.
AU - J. G. Laird
AU - J. Soetedjo
AU - S. M. Inamdar
AU - A. R. Bock
AU - E. S. Ataman
AU - M. A. Pufall
AU - B. A. Berkowitz
AU - S. A. Baker
PY - 2026
DA - 2026/06/27
PB - bioRxiv
DO - 10.64898/2026.06.27.734996
UR - https://www.biorxiv.org/content/10.64898/2026.06.27.734996v2
AB - Purpose: Photoreceptor Kv2.1/Kv8.2 voltage-gated potassium channels carry an outward potassium current, helping to set the resting membrane potential and to shape dim light responses. Because potassium flux in the outer retina influences extracellular osmolarity and fluid distribution, we hypothesized that Kv2.1/Kv8.2 channels also contribute to fluid homeostasis in this region of the retina. Methods: OCT imaging was performed in Kv8.2 heterozygous (Het) and knockout (KO) mice aged ~5 weeks under dark- and light-adapted conditions. Light-dark differences in the distance between the external limiting membrane (ELM) and retinal pigment epithelium (RPE) ({Delta}ELM-RPE) were calculated to quantify light-evoked outer retinal expansion, a biomarker associated with changes in subretinal space (SRS) fluid handling. As a secondary outcome, outer nuclear layer (ONL) thickness was also measured under both lighting conditions. Retinal gene expression was assessed by RNA-seq and droplet digital RT-PCR. Retinal protein expression was determined by western blotting and immunolabeling. Results: {Delta}ELM-RPE was significantly reduced in Kv8.2 KO mice compared with Het controls, indicating altered light-evoked outer retinal dynamics compatible with changes in subretinal space fluid handling. ONL thickness exhibited a small but significant light-dark change that was different between genotypes. Transcriptomic analyses revealed upregulation of osmosensitive genes, including osmolyte transporters and aquaporins. AQP1 protein expression in photoreceptors increased. Conclusions: These findings are consistent with altered outer retinal fluid homeostasis following loss of Kv8.2 and support a model in which photoreceptor potassium efflux contributes to osmotic water movement into the subretinal space.
ER -