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Peer-reviewedCC BY-NC 4.0microLife

A putative type IVB secretion system contributes to the pathogenicity of Yersinia pseudotuberculosis lineage 8 strains associated with the Far East scarlet-like fever

  • Marion Lemarignier1
  • Cyril Savin1,2
  • Inés Ruedas-Torres3
  • Anne Derbise1
  • Charles Coluzzi4
  • Jose Pablo Marin-Obando5
  • Josue Barquero-Chavarria1
  • Pierre Lê-Bury1
  • Julien Burlaud-Gaillard6
  • Julien Madej1,2
  • Rémi Beau1,2
  • Philippe Roingeard6
4 more authors
  • Pierre Lechat7
  • Eduardo P C Rocha4
  • Jaime Gómez-Laguna8
  • Javier Pizarro-Cerdá1,2
8 affiliations
  1. 1Yersinia Research Unit, Institut Pasteur, Université Paris Cité, CNRS UMR 6047, F-75015 Paris, France.
  2. 2Yersinia National Reference Laboratory, WHO Collaborating Research & Reference Centre for Plague FRA-146, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.
  3. 3United Kingdom Health Security Agency (UKHSA), Porton Down, SP4 0JG Salisbury, United Kingdom.
  4. 4Microbial Evolutionary Genomics, Institut Pasteur, Université Paris Cité, CNRS UMR 3525, F-75015 Paris, France.
  5. 5Environment and Infectious Risks Unit, Laboratory for Urgent Response to Biological Threats, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.
  6. 6IBiSA Electron Microscopy Platform, INSERM U1259, Université de Tours et CHRU de Tours, F-37032 Tours, France.
  7. 7Bioinformatics and Biostatistics Hub, Institut Pasteur, Université Paris Cité, F-75015 Paris, France.
  8. 8Department of Anatomy and Comparative Pathology and Toxicology, Pathology and Immunology Group (UCO-PIG), UIC Zoonosis y Enfermedades Emergentes ENZOEM, International Excellence Agrifood Campus 'CeiA3', University of Córdoba, 14014 Córdoba, Spain.
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Abstract

Yersinia pseudotuberculosis is a food-borne pathogen responsible for a self-limiting mesenteric lymphadenitis. Yersinia pseudotuberculosis lineages 1 and 8 are also associated to the Far East scarlet-like fever (FESLF) syndrome, characterized by skin rash, hyperemic tongue, and desquamation. Genome sequencing of lineage 8 FESLF strains previously revealed the presence in the plasmid pVM82 of dot/icm genes, homologous to those known to encode a Type 4B Secretion System (T4BSS) in the intracellular pathogens Legionella pneumophila and Coxiella burnetii . We validated the presence of all essential dot/icm genes required for the structure of a T4BSS and we identified acidic pH and 37 • C as the main signals triggering y T4BSS expression. Putative y T4BSS effectors were also identified using the S4TE algorithm but their secretion was not confirmed in the studied conditions. The y T4BSS is also expressed in epithelial cells and macrophages as well as in vivo during mouse infection, but it is not required for bacterial intracellular survival. Nevertheless, the y T4BSS is implicated in Y. pseudotuberculosis FESLF pathogenicity in mice when bacteria are orally inoculated. The y T4BSS does not influence organ colonization and is rather implicated in the induction of important necrosis lesions in mesenteric lymph nodes and ceca of mice. Cytokine profile analyses in mouse bone marrow-derived macrophages revealed a y T4BSS-dependent induction of innate immunity related cytokines and chemokines. In conclusion, we identify the y T4BSS as a virulence factor of Y. pseudotuberculosis strains from lineage 8 responsible for FESLF which modulates cytokine responses of the host innate immune system.

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Cite

BibTeXRIS
BibTeX
@article{lemarignier2026putative,
  title = {{A putative type IVB secretion system contributes to the pathogenicity of Yersinia pseudotuberculosis lineage 8 strains associated with the Far East scarlet-like fever}},
  author = {Marion Lemarignier and Cyril Savin and Inés Ruedas-Torres and Anne Derbise and Charles Coluzzi and Jose Pablo Marin-Obando and Josue Barquero-Chavarria and Pierre Lê-Bury and Julien Burlaud-Gaillard and Julien Madej and Rémi Beau and Philippe Roingeard and Pierre Lechat and Eduardo P C Rocha and Jaime Gómez-Laguna and Javier Pizarro-Cerdá},
  journal = {microLife},
  year = {2026},
  month = sep,
  doi = {10.1093/femsml/uqag036},
  url = {https://pasteur.hal.science/pasteur-05800334v1},
  note = {CC BY-NC 4.0},
}
RIS
TY  - JOUR
TI  - A putative type IVB secretion system contributes to the pathogenicity of Yersinia pseudotuberculosis lineage 8 strains associated with the Far East scarlet-like fever
AU  - Marion Lemarignier
AU  - Cyril Savin
AU  - Inés Ruedas-Torres
AU  - Anne Derbise
AU  - Charles Coluzzi
AU  - Jose Pablo Marin-Obando
AU  - Josue Barquero-Chavarria
AU  - Pierre Lê-Bury
AU  - Julien Burlaud-Gaillard
AU  - Julien Madej
AU  - Rémi Beau
AU  - Philippe Roingeard
AU  - Pierre Lechat
AU  - Eduardo P C Rocha
AU  - Jaime Gómez-Laguna
AU  - Javier Pizarro-Cerdá
PY  - 2026
DA  - 2026/09/23
JO  - microLife
DO  - 10.1093/femsml/uqag036
UR  - https://pasteur.hal.science/pasteur-05800334v1
AB  - Yersinia pseudotuberculosis is a food-borne pathogen responsible for a self-limiting mesenteric lymphadenitis. Yersinia pseudotuberculosis lineages 1 and 8 are also associated to the Far East scarlet-like fever (FESLF) syndrome, characterized by skin rash, hyperemic tongue, and desquamation. Genome sequencing of lineage 8 FESLF strains previously revealed the presence in the plasmid pVM82 of dot/icm genes, homologous to those known to encode a Type 4B Secretion System (T4BSS) in the intracellular pathogens Legionella pneumophila and Coxiella burnetii . We validated the presence of all essential dot/icm genes required for the structure of a T4BSS and we identified acidic pH and 37 • C as the main signals triggering y T4BSS expression. Putative y T4BSS effectors were also identified using the S4TE algorithm but their secretion was not confirmed in the studied conditions. The y T4BSS is also expressed in epithelial cells and macrophages as well as in vivo during mouse infection, but it is not required for bacterial intracellular survival. Nevertheless, the y T4BSS is implicated in Y. pseudotuberculosis FESLF pathogenicity in mice when bacteria are orally inoculated. The y T4BSS does not influence organ colonization and is rather implicated in the induction of important necrosis lesions in mesenteric lymph nodes and ceca of mice. Cytokine profile analyses in mouse bone marrow-derived macrophages revealed a y T4BSS-dependent induction of innate immunity related cytokines and chemokines. In conclusion, we identify the y T4BSS as a virulence factor of Y. pseudotuberculosis strains from lineage 8 responsible for FESLF which modulates cytokine responses of the host innate immune system.
SN  - 2633-6693
ER  -