The misplaced mouse Pax6 interneuron subclass: A cross-species transcriptomic reassignment.
- 1Physiology Department and Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
Abstract
Subclass cell types are strongly conserved across mammalian brains, making the systemic absence of a caudal ganglionic eminence (CGE) Pax6 interneuron subclass in mouse transcriptional atlases unexpected. Through cross-species transcriptomic analysis, we identify a Pax6 subclass homolog in mouse and uncover primate-specific divergence in the Sncg subclass. Analyses of electrophysiological and spatial datasets reveal limited subclass-specific structure in either modality, emphasizing the superior resolving power of transcriptomics for subclass distinctions. Our results highlight the pitfalls of relying on small sets of marker genes in cell-type annotation and provide evolution-aware annotations and marker sets to support robust cross-mammal interneuron comparisons.
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BibTeX
@article{werner2026misplaced,
title = {{The misplaced mouse Pax6 interneuron subclass: A cross-species transcriptomic reassignment.}},
author = {Jonathan M Werner and Hamsini Suresh and Leon French and Jesse Gillis},
journal = {IScience},
year = {2026},
month = sep,
doi = {10.1016/j.isci.2026.117632},
url = {https://europepmc.org/article/MED/42840470},
note = {CC BY},
}
RIS
TY - JOUR
TI - The misplaced mouse Pax6 interneuron subclass: A cross-species transcriptomic reassignment.
AU - Jonathan M Werner
AU - Hamsini Suresh
AU - Leon French
AU - Jesse Gillis
PY - 2026
DA - 2026/09/26
JO - IScience
DO - 10.1016/j.isci.2026.117632
UR - https://europepmc.org/article/MED/42840470
AB - Subclass cell types are strongly conserved across mammalian brains, making the systemic absence of a caudal ganglionic eminence (CGE) Pax6 interneuron subclass in mouse transcriptional atlases unexpected. Through cross-species transcriptomic analysis, we identify a Pax6 subclass homolog in mouse and uncover primate-specific divergence in the Sncg subclass. Analyses of electrophysiological and spatial datasets reveal limited subclass-specific structure in either modality, emphasizing the superior resolving power of transcriptomics for subclass distinctions. Our results highlight the pitfalls of relying on small sets of marker genes in cell-type annotation and provide evolution-aware annotations and marker sets to support robust cross-mammal interneuron comparisons.
SN - 2589-0042
ER -