Effects of Cd8a Gene Knockout on Gut Microbiota and Liver Metabolism.
4 affiliations
- 1School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, China.
- 2Key Laboratory of Common Infectious Disease Prevention and Control in Ningxia, Yinchuan, Ningxia, China.
- 3School of Laboratory Medicine, Ningxia Medical University, Yinchuan, Ningxia, China.
- 4General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Abstract
Objective
Evaluate the effects of CD8⁺ T cell defects caused by Cd8a gene knockout on gut microbiota and hepatic metabolism, and preliminarily explore the underlying mechanisms.Methods
The Cd8a gene was knocked out in mice to generate Cd8a knockout mice. Genotypes were identified using polymerase chain reaction (PCR) and agarose gel electrophoresis. Mice were divided into three groups: normal control, CD8⁺ T cell-deficient, and CD8⁺ T cell reinfusion, with six mice per group. CD8⁺ T cells from normal control mice were isolated via magnetic bead sorting and reinfused into CD8⁺ T cell-deficient mice via tail vein injection. Reinfusions were performed four times at 3-day intervals. Four weeks after the final reinfusion, fecal samples were collected, and DNA extracted for 16S rRNA gene sequencing analysis. Mouse livers were collected and analyzed using non-targeted metabolomics via liquid chromatography-tandem mass spectrometry (LC-MS/MS).Results
We found that CD8⁺ T cell deficiency-induced alterations in gut microbiota primarily affected the phyla Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteriota. Following CD8⁺ T cell depletion, the abundance of bacteria such as Catabacter, Vicinamibacter, Mesorhizobium, Catellatospora, and Stomatobaculum increased (p < 0.05); after CD8⁺ T cell reinfusion, the abundance of these bacteria showed a decreasing trend (p < 0.05). We also identified five major metabolic pathways in mouse liver that showed significant correlations with CD8⁺ T cell status.Conclusion
CD8⁺ T cells exert a significant influence on hepatic metabolic responses and gut microbial community diversity.
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BibTeX
@article{dai2026effects,
title = {{Effects of Cd8a Gene Knockout on Gut Microbiota and Liver Metabolism.}},
author = {Zhongshi Dai and Chuan Wang and Hu Mu and Chunxiu Hou and Jing Tang and Jinhai Ma and Mingxing Zhu and Bingshuo Qian},
journal = {Immunity, inflammation and disease},
year = {2026},
month = oct,
doi = {10.1002/iid3.70557},
url = {https://europepmc.org/article/MED/42885142},
note = {CC BY},
}
RIS
TY - JOUR
TI - Effects of Cd8a Gene Knockout on Gut Microbiota and Liver Metabolism.
AU - Zhongshi Dai
AU - Chuan Wang
AU - Hu Mu
AU - Chunxiu Hou
AU - Jing Tang
AU - Jinhai Ma
AU - Mingxing Zhu
AU - Bingshuo Qian
PY - 2026
DA - 2026/10/01
JO - Immunity, inflammation and disease
DO - 10.1002/iid3.70557
UR - https://europepmc.org/article/MED/42885142
AB - Objective Evaluate the effects of CD8⁺ T cell defects caused by Cd8a gene knockout on gut microbiota and hepatic metabolism, and preliminarily explore the underlying mechanisms.Methods The Cd8a gene was knocked out in mice to generate Cd8a knockout mice. Genotypes were identified using polymerase chain reaction (PCR) and agarose gel electrophoresis. Mice were divided into three groups: normal control, CD8⁺ T cell-deficient, and CD8⁺ T cell reinfusion, with six mice per group. CD8⁺ T cells from normal control mice were isolated via magnetic bead sorting and reinfused into CD8⁺ T cell-deficient mice via tail vein injection. Reinfusions were performed four times at 3-day intervals. Four weeks after the final reinfusion, fecal samples were collected, and DNA extracted for 16S rRNA gene sequencing analysis. Mouse livers were collected and analyzed using non-targeted metabolomics via liquid chromatography-tandem mass spectrometry (LC-MS/MS).Results We found that CD8⁺ T cell deficiency-induced alterations in gut microbiota primarily affected the phyla Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteriota. Following CD8⁺ T cell depletion, the abundance of bacteria such as Catabacter, Vicinamibacter, Mesorhizobium, Catellatospora, and Stomatobaculum increased (p < 0.05); after CD8⁺ T cell reinfusion, the abundance of these bacteria showed a decreasing trend (p < 0.05). We also identified five major metabolic pathways in mouse liver that showed significant correlations with CD8⁺ T cell status.Conclusion CD8⁺ T cells exert a significant influence on hepatic metabolic responses and gut microbial community diversity.
SN - 2050-4527
ER -