Polysaccharides from Radix Hedysari exhibited preventive effects against DSS-induced ulcerative colitis in C57BL/6 mice
- 1Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College , ,
Abstract
Hedysarum polysaccharide (HPS), the major bioactive constituent of Radix Hedysari, possesses anti-inflammatory activity; however, few studies have explored its application for ulcerative colitis (UC). In this study, HPS-80 was extracted using combined complex-enzyme hydrolysis and ultrasonication. We further evaluated its preventive effects and underlying mechanisms in a dextran sulfate sodium (DSS)-induced UC model in C57BL/6 mice. Results showed HPS-80 significantly improved DSS-induced UC symptoms (weight loss, hematochezia, colon shortening) and reduced Disease Activity Index (DAI). Mechanistically, it inhibited pro-inflammatory factors Tumor Necrosis Factor-alpha(TNF-α),Interleukin-6, (IL-6), alleviated oxidative stress, upregulated tight junction proteins occludin, claudin-1(ZO-1), and modulated intestinal microbiota (suppressed harmful bacteria like Bacteroidota, enriched beneficial ones like Firmicutes and Akkermansia). Conclusion: HPS-80 exerts protective effects against UC via anti-inflammatory, antioxidant and gut-microbiota-regulating capacities. It is a promising candidate agent for UC prevention.
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BibTeX
@article{ma2026polysaccharides,
title = {{Polysaccharides from Radix Hedysari exhibited preventive effects against DSS-induced ulcerative colitis in C57BL/6 mice}},
author = {Lujie Ma and Yu Han and Xueqin Zhao and Yixiu Niu and Yuxin Xie and Yating Pan and Yaxin Zheng and Zhiyuan Xue},
journal = {Frontiers in immunology},
year = {2026},
month = oct,
doi = {10.3389/fimmu.2026.1944924},
url = {https://europepmc.org/article/PMC/PMC13673562},
note = {CC BY},
}
RIS
TY - JOUR
TI - Polysaccharides from Radix Hedysari exhibited preventive effects against DSS-induced ulcerative colitis in C57BL/6 mice
AU - Lujie Ma
AU - Yu Han
AU - Xueqin Zhao
AU - Yixiu Niu
AU - Yuxin Xie
AU - Yating Pan
AU - Yaxin Zheng
AU - Zhiyuan Xue
PY - 2026
DA - 2026/10/02
JO - Frontiers in immunology
DO - 10.3389/fimmu.2026.1944924
UR - https://europepmc.org/article/PMC/PMC13673562
AB - Hedysarum polysaccharide (HPS), the major bioactive constituent of Radix Hedysari, possesses anti-inflammatory activity; however, few studies have explored its application for ulcerative colitis (UC). In this study, HPS-80 was extracted using combined complex-enzyme hydrolysis and ultrasonication. We further evaluated its preventive effects and underlying mechanisms in a dextran sulfate sodium (DSS)-induced UC model in C57BL/6 mice. Results showed HPS-80 significantly improved DSS-induced UC symptoms (weight loss, hematochezia, colon shortening) and reduced Disease Activity Index (DAI). Mechanistically, it inhibited pro-inflammatory factors Tumor Necrosis Factor-alpha(TNF-α),Interleukin-6, (IL-6), alleviated oxidative stress, upregulated tight junction proteins occludin, claudin-1(ZO-1), and modulated intestinal microbiota (suppressed harmful bacteria like Bacteroidota, enriched beneficial ones like Firmicutes and Akkermansia). Conclusion: HPS-80 exerts protective effects against UC via anti-inflammatory, antioxidant and gut-microbiota-regulating capacities. It is a promising candidate agent for UC prevention.
SN - 1664-3224
ER -