MRSA‐Triggered Disassembly of an Immune‐Recruiting NIR Probe for Bacterial Imaging and Clearance
- 1State Key Laboratory of Bioactive Substances and Function of Natural Medicine Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China
Abstract
Methicillin‐resistant Staphylococcus aureus (MRSA) infections remain a major clinical challenge due to increasing antimicrobial resistance and limited strategies for precise bacterial recognition and clearance. Here, we report an immune‐recruiting supramolecular amphiphile, Dman‐C11, that undergoes MRSA‐triggered structural reorganization for activatable bacterial theranostics. Dman‐C11 consists of a hemicyanine near‐infrared (NIR) fluorophore, a D‐mannosamine immune‐recruiting motif, and a hydrophobic C11 chain that promotes bacterial membrane association. The amphiphilic architecture drives spontaneous nanoparticle formation with suppressed fluorescence through aggregation‐caused quenching (ACQ). Upon interaction with the MRSA membrane, nanoparticle disassembly redistributes the probe within the lipid bilayer, producing a 20‐fold fluorescence turn‐on response while simultaneously restoring efficient singlet oxygen generation under 655 nm irradiation. The activated photodynamic process induces ferroptosis‐like bacterial death characterized by extensive lipid peroxidation and oxidative membrane damage. Meanwhile, the D‐mannosamine motif promotes immune‐cell recruitment toward bacterial pathogens, thereby facilitating host‐assisted bacterial clearance. In murine wound infection and bacterial keratitis models, Dman‐C11 achieves approximately 20‐fold reductions in bacterial burden without detectable systemic toxicity. This membrane‐triggered supramolecular disassembly strategy establishes a single‐molecule theranostic approach that links bacterial recognition, activatable imaging, photodynamic antibacterial activity, and immune‐assisted clearance for precise treatment of MRSA infections.
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BibTeX
@article{du2026mrsatriggered,
title = {{MRSA‐Triggered Disassembly of an Immune‐Recruiting NIR Probe for Bacterial Imaging and Clearance}},
author = {Jiacheng Du and Yongyi Chen and Ling Lei and Yunzhe Tao and Yu Peng and Qingyang Zhang and Qinghua Wang and Hai‐Yu Hu},
journal = {Advanced Science},
year = {2026},
month = oct,
doi = {10.1002/advs.78522},
url = {https://doi.org/10.1002/advs.78522},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - MRSA‐Triggered Disassembly of an Immune‐Recruiting NIR Probe for Bacterial Imaging and Clearance
AU - Jiacheng Du
AU - Yongyi Chen
AU - Ling Lei
AU - Yunzhe Tao
AU - Yu Peng
AU - Qingyang Zhang
AU - Qinghua Wang
AU - Hai‐Yu Hu
PY - 2026
DA - 2026/10/31
JO - Advanced Science
DO - 10.1002/advs.78522
UR - https://doi.org/10.1002/advs.78522
AB - Methicillin‐resistant Staphylococcus aureus (MRSA) infections remain a major clinical challenge due to increasing antimicrobial resistance and limited strategies for precise bacterial recognition and clearance. Here, we report an immune‐recruiting supramolecular amphiphile, Dman‐C11, that undergoes MRSA‐triggered structural reorganization for activatable bacterial theranostics. Dman‐C11 consists of a hemicyanine near‐infrared (NIR) fluorophore, a D‐mannosamine immune‐recruiting motif, and a hydrophobic C11 chain that promotes bacterial membrane association. The amphiphilic architecture drives spontaneous nanoparticle formation with suppressed fluorescence through aggregation‐caused quenching (ACQ). Upon interaction with the MRSA membrane, nanoparticle disassembly redistributes the probe within the lipid bilayer, producing a 20‐fold fluorescence turn‐on response while simultaneously restoring efficient singlet oxygen generation under 655 nm irradiation. The activated photodynamic process induces ferroptosis‐like bacterial death characterized by extensive lipid peroxidation and oxidative membrane damage. Meanwhile, the D‐mannosamine motif promotes immune‐cell recruitment toward bacterial pathogens, thereby facilitating host‐assisted bacterial clearance. In murine wound infection and bacterial keratitis models, Dman‐C11 achieves approximately 20‐fold reductions in bacterial burden without detectable systemic toxicity. This membrane‐triggered supramolecular disassembly strategy establishes a single‐molecule theranostic approach that links bacterial recognition, activatable imaging, photodynamic antibacterial activity, and immune‐assisted clearance for precise treatment of MRSA infections.
SN - 2198-3844
ER -