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Peer-reviewedCC BY-NC-NDEnvironment & health (Washington, D.C.)

CRISPR Technology Applied to the Analysis of Antibiotic Resistance Genes in the Environment.

  • Jiayin Yan1,2
  • Xiaoxian Yuan1,3,4
  • Zijun Zhu1,3,4
  • Zhuang Li1,3,4
  • Minghuo Wu2
  • Jing Liu1,3
  • Lizhong Zhu1,3,4
  • Wei Feng1,3,4
4 affiliations
  1. 1State Key Laboratory of Soil Pollution Control and Safety, Zhejiang University, Hangzhou 310058, China.
  2. 2School of Chemical Engineering, Ocean and Life Science, Dalian University of Technology, Panjin 124221, China.
  3. 3College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
  4. 4Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China.
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Abstract

Environmental transmission of antibiotic resistance poses a risk to human health. Understanding the behavior and health risks of antibiotic resistance genes (ARGs) in the environment requires accurate nucleic acid analyses. Technical challenges remain in the sequencing of ARGs in low abundance relative to total environmental DNA and in on-site analysis. CRISPR technology has been applied to the characterization and monitoring of ARGs in various environmental media. For sequencing of ARGs, CRISPR technology has enabled the enrichment of ARGs present in the environment at a very low abundance. Using CRISPR/Cas9 systems with a pool of guide RNAs (gRNAs) could simultaneously enrich a large panel of ARGs in one environmental sample before sequencing for good coverage and reading depth. CRISPR/Cas9 also enabled targeted long-read sequencing for understanding the genetic context of low-abundance ARGs. CRISPR-based rapid detection has the potential for on-site analysis. Current applications include the analysis of antibiotic-resistant bacteria (ARB) and ARGs in the air and the urban water cycle. CRISPR technology is also useful for differentiating single-nucleotide mutations between antibiotic-susceptible and -resistant variants. Rapid detection of antibiotics together with ARGs is also possible with the use of aptamers that specifically bind to antibiotics.

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Cite

BibTeXRIS
BibTeX
@article{yan2026crispr,
  title = {{CRISPR Technology Applied to the Analysis of Antibiotic Resistance Genes in the Environment.}},
  author = {Jiayin Yan and Xiaoxian Yuan and Zijun Zhu and Zhuang Li and Minghuo Wu and Jing Liu and Lizhong Zhu and Wei Feng},
  journal = {Environment \& health (Washington, D.C.)},
  year = {2026},
  month = jun,
  doi = {10.1021/envhealth.5c00855},
  url = {https://europepmc.org/article/MED/42903801},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - CRISPR Technology Applied to the Analysis of Antibiotic Resistance Genes in the Environment.
AU  - Jiayin Yan
AU  - Xiaoxian Yuan
AU  - Zijun Zhu
AU  - Zhuang Li
AU  - Minghuo Wu
AU  - Jing Liu
AU  - Lizhong Zhu
AU  - Wei Feng
PY  - 2026
DA  - 2026/06/05
JO  - Environment & health (Washington, D.C.)
DO  - 10.1021/envhealth.5c00855
UR  - https://europepmc.org/article/MED/42903801
AB  - Environmental transmission of antibiotic resistance poses a risk to human health. Understanding the behavior and health risks of antibiotic resistance genes (ARGs) in the environment requires accurate nucleic acid analyses. Technical challenges remain in the sequencing of ARGs in low abundance relative to total environmental DNA and in on-site analysis. CRISPR technology has been applied to the characterization and monitoring of ARGs in various environmental media. For sequencing of ARGs, CRISPR technology has enabled the enrichment of ARGs present in the environment at a very low abundance. Using CRISPR/Cas9 systems with a pool of guide RNAs (gRNAs) could simultaneously enrich a large panel of ARGs in one environmental sample before sequencing for good coverage and reading depth. CRISPR/Cas9 also enabled targeted long-read sequencing for understanding the genetic context of low-abundance ARGs. CRISPR-based rapid detection has the potential for on-site analysis. Current applications include the analysis of antibiotic-resistant bacteria (ARB) and ARGs in the air and the urban water cycle. CRISPR technology is also useful for differentiating single-nucleotide mutations between antibiotic-susceptible and -resistant variants. Rapid detection of antibiotics together with ARGs is also possible with the use of aptamers that specifically bind to antibiotics.
SN  - 2833-8278
ER  -