Development of a cross-protective subunit cocktail vaccine against diverse serotypes of Glaesserella parasuis infection in pigs
Abstract
Glässer’s disease, caused by Glaesserella parasuis (GPS), causes substantial economic losses in the swine industry worldwide. This situation is largely attributable to the poor cross-protective efficacy of currently available vaccines. In this study, a multi-antigen cocktail subunit vaccine against GPS infection was developed. In silico antigenicity analysis, the immunoprotective efficacy, and antibody titers of 14 GPS antigens were assessed in a murine model. Finally, VacJ, PtsG, and GAPDH were identified as the component of the cocktail vaccine, with Gel-01 as the optimal adjuvant. Immunization of the cocktail vaccine induced robust humoral and cellular immune responses. The vaccine conferred effective cross-protection against lethal-dose GPS4 and GPS5 infections in mice. As supported by a pilot study, it also demonstrated favorable immunoprotective efficacy against GPS infection in piglets. Furthermore, immune sera from vaccinated mice significantly inhibited the growth of GPS4 and GPS5. Overall, these results suggested that the subunit cocktail vaccine established in this study exhibits great potential for preventing GPS infection under experimental conditions, and further large‑scale pig trials are still required to validate its practical applicability in field production.
Related
- IFN-λ fine tunes protective immunity during mucosal viral infection.
- CX3CR1+ microglia/macrophages, activated T cells, and IFN-γ-driven stimulation confer age-dependent protective immunity in young-adult mice following β-coronavirus infection.
- Development of an edible vaccine candidate conferring protective efficacy against pneumococcal challenge using conserved PspA epitopes in a murine model
- CD4+ T cell-intrinsic T-bet sustains protective CNS immunity and parasite control during Toxoplasma gondii infection
- L-Serine and Palmitoyl CoA control Mycobacterial infection by tweaking protective immune response: A potential host directed therapy for tuberculosis
BibTeX
@article{li2026development,
title = {{Development of a cross-protective subunit cocktail vaccine against diverse serotypes of Glaesserella parasuis infection in pigs}},
author = {Fengyang Li and Yan Gong and Ziheng Li and Zhen Wang and Zengshuai Wu and Di Zhang and Peng Zhang and Zhichao Lu and Hong Chu and Kaixin Zhang and Diansong Li and Na Li and Liancheng Lei},
journal = {Veterinary Research},
year = {2026},
month = sep,
doi = {10.1186/s13567-026-01846-3},
url = {https://hal.science/hal-05766456v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Development of a cross-protective subunit cocktail vaccine against diverse serotypes of Glaesserella parasuis infection in pigs
AU - Fengyang Li
AU - Yan Gong
AU - Ziheng Li
AU - Zhen Wang
AU - Zengshuai Wu
AU - Di Zhang
AU - Peng Zhang
AU - Zhichao Lu
AU - Hong Chu
AU - Kaixin Zhang
AU - Diansong Li
AU - Na Li
AU - Liancheng Lei
PY - 2026
DA - 2026/09/28
JO - Veterinary Research
DO - 10.1186/s13567-026-01846-3
UR - https://hal.science/hal-05766456v1
AB - Glässer’s disease, caused by Glaesserella parasuis (GPS), causes substantial economic losses in the swine industry worldwide. This situation is largely attributable to the poor cross-protective efficacy of currently available vaccines. In this study, a multi-antigen cocktail subunit vaccine against GPS infection was developed. In silico antigenicity analysis, the immunoprotective efficacy, and antibody titers of 14 GPS antigens were assessed in a murine model. Finally, VacJ, PtsG, and GAPDH were identified as the component of the cocktail vaccine, with Gel-01 as the optimal adjuvant. Immunization of the cocktail vaccine induced robust humoral and cellular immune responses. The vaccine conferred effective cross-protection against lethal-dose GPS4 and GPS5 infections in mice. As supported by a pilot study, it also demonstrated favorable immunoprotective efficacy against GPS infection in piglets. Furthermore, immune sera from vaccinated mice significantly inhibited the growth of GPS4 and GPS5. Overall, these results suggested that the subunit cocktail vaccine established in this study exhibits great potential for preventing GPS infection under experimental conditions, and further large‑scale pig trials are still required to validate its practical applicability in field production.
SN - 0928-4249
SN - 1297-9716
ER -