Analysis of Metabolic Differences Between Marselan and Cabernet Sauvignon Grape Berries from the Manas Wine-Producing Region of Xinjiang Based on Untargeted Metabolomics.
- 1College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China.
Abstract
To characterize cultivar-specific metabolic differences between Marselan and Cabernet Sauvignon grapes cultivated in the Manas subregion on the northern foothills of the Tianshan Mountains in Xinjiang, China, mature berries from the two cultivars grown in adjacent plots within the same vineyard were analyzed, with three independent biological replicates per cultivar (n = 3). Untargeted metabolomics based on UHPLC-Q Exactive MS was used to compare the overall metabolic profiles of the two cultivars. PCA revealed clear separation between their global metabolic profiles in both positive- and negative-ion modes. A total of 236 candidate differential metabolites were identified using VIP > 1 and unadjusted p < 0.05, of which 34 further met FDR ≤ 0.05 and |log₂FC| ≥ 1. After reviewing metabolite annotation evidence and grape berry metabolic context, 13 biologically interpretable features were selected for hierarchical clustering in the main text. KEGG analysis showed that flavonoid biosynthesis remained significantly enriched after multiple-testing correction, whereas phenylalanine metabolism and flavone and flavonol biosynthesis also showed distinct differential metabolite mapping patterns. Overall, Marselan and Cabernet Sauvignon exhibited distinct cultivar-associated metabolic phenotypes in mature berries, mainly involving phenylalanine and its downstream phenolic and flavonoid-related metabolic processes. Given the limited sample size and the risk of overfitting in supervised models, the conclusions were interpreted mainly on the basis of PCA, multiple-testing-corrected univariate statistics, and metabolite annotation evidence.
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BibTeX
@article{wei2026analysis,
title = {{Analysis of Metabolic Differences Between Marselan and Cabernet Sauvignon Grape Berries from the Manas Wine-Producing Region of Xinjiang Based on Untargeted Metabolomics.}},
author = {Guiyin Wei and Yun Wu and Na Xu and Jiawei Zhu and Ying Deng and Tiancong Zheng and Wenshu Huang and Weilin Chen and Wenrui Ma},
journal = {Foods (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/foods15193534},
url = {https://europepmc.org/article/MED/42883643},
note = {CC BY},
}
RIS
TY - JOUR
TI - Analysis of Metabolic Differences Between Marselan and Cabernet Sauvignon Grape Berries from the Manas Wine-Producing Region of Xinjiang Based on Untargeted Metabolomics.
AU - Guiyin Wei
AU - Yun Wu
AU - Na Xu
AU - Jiawei Zhu
AU - Ying Deng
AU - Tiancong Zheng
AU - Wenshu Huang
AU - Weilin Chen
AU - Wenrui Ma
PY - 2026
DA - 2026/10/03
JO - Foods (Basel, Switzerland)
DO - 10.3390/foods15193534
UR - https://europepmc.org/article/MED/42883643
AB - To characterize cultivar-specific metabolic differences between Marselan and Cabernet Sauvignon grapes cultivated in the Manas subregion on the northern foothills of the Tianshan Mountains in Xinjiang, China, mature berries from the two cultivars grown in adjacent plots within the same vineyard were analyzed, with three independent biological replicates per cultivar (n = 3). Untargeted metabolomics based on UHPLC-Q Exactive MS was used to compare the overall metabolic profiles of the two cultivars. PCA revealed clear separation between their global metabolic profiles in both positive- and negative-ion modes. A total of 236 candidate differential metabolites were identified using VIP > 1 and unadjusted p < 0.05, of which 34 further met FDR ≤ 0.05 and |log₂FC| ≥ 1. After reviewing metabolite annotation evidence and grape berry metabolic context, 13 biologically interpretable features were selected for hierarchical clustering in the main text. KEGG analysis showed that flavonoid biosynthesis remained significantly enriched after multiple-testing correction, whereas phenylalanine metabolism and flavone and flavonol biosynthesis also showed distinct differential metabolite mapping patterns. Overall, Marselan and Cabernet Sauvignon exhibited distinct cultivar-associated metabolic phenotypes in mature berries, mainly involving phenylalanine and its downstream phenolic and flavonoid-related metabolic processes. Given the limited sample size and the risk of overfitting in supervised models, the conclusions were interpreted mainly on the basis of PCA, multiple-testing-corrected univariate statistics, and metabolite annotation evidence.
SN - 2304-8158
ER -