Patching up the nucleus: a novel role for PMLII in nuclear envelope stability
Abstract
The nuclear envelope (NE) is important for cellular health as it protects and organizes the genome. NE dynamics are important for various cellular processes, including cell growth, migration, and removal of defective NE components. In extreme cases, the NE can rupture, followed by rapid repair to minimize damage to the genome. While our understanding of the repair process is increasing, a lot is still unknown about events leading up to NE rupture. Here, we found that promyelocytic leukemia protein isoform II (PMLII), a protein involved in nuclear PML body formation, forms condensates at the NE. PML condensates specifically form at sites where the lamina is disrupted, and two predicted amphipathic α-helices in the unique C-terminus of PMLII are essential for this localization. Live-cell imaging showed that when NE rupture occurs at these sites, the PMLII patch remains present until rupture repair is initiated. Using stable cell lines expressing FLAG-PMLII in a PML knockdown background, we found that PMLII caused an increase in the size of lamin holes without increasing the number of NE ruptures. We therefore hypothesize that PML patches play a role in NE integrity maintenance and dynamics through the stabilization of large holes in the lamina.
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BibTeX
@article{janssen2026patching,
title = {{Patching up the nucleus: a novel role for PMLII in nuclear envelope stability}},
author = {Anne F J Janssen and Oliver Knowles and Sébastien Britton and Janet R Kumita and Janet E Deane and Evan Spruijt and Delphine Larrieu},
journal = {Nucleic Acids Research},
year = {2026},
month = jul,
doi = {10.1093/nar/gkag665},
url = {https://cnrs.hal.science/hal-05772766v1},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - Patching up the nucleus: a novel role for PMLII in nuclear envelope stability
AU - Anne F J Janssen
AU - Oliver Knowles
AU - Sébastien Britton
AU - Janet R Kumita
AU - Janet E Deane
AU - Evan Spruijt
AU - Delphine Larrieu
PY - 2026
DA - 2026/07/17
JO - Nucleic Acids Research
DO - 10.1093/nar/gkag665
UR - https://cnrs.hal.science/hal-05772766v1
AB - The nuclear envelope (NE) is important for cellular health as it protects and organizes the genome. NE dynamics are important for various cellular processes, including cell growth, migration, and removal of defective NE components. In extreme cases, the NE can rupture, followed by rapid repair to minimize damage to the genome. While our understanding of the repair process is increasing, a lot is still unknown about events leading up to NE rupture. Here, we found that promyelocytic leukemia protein isoform II (PMLII), a protein involved in nuclear PML body formation, forms condensates at the NE. PML condensates specifically form at sites where the lamina is disrupted, and two predicted amphipathic α-helices in the unique C-terminus of PMLII are essential for this localization. Live-cell imaging showed that when NE rupture occurs at these sites, the PMLII patch remains present until rupture repair is initiated. Using stable cell lines expressing FLAG-PMLII in a PML knockdown background, we found that PMLII caused an increase in the size of lamin holes without increasing the number of NE ruptures. We therefore hypothesize that PML patches play a role in NE integrity maintenance and dynamics through the stabilization of large holes in the lamina.
SN - 0305-1048
SN - 1362-4962
ER -