Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin
- 1Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China
Abstract
We present a time-series single-nucleus ATAC-seq dataset from full-thickness excisional wounds in Sprague-Dawley rats. Samples were taken from unwounded skin (normal control) and at 1, 2, 5, 10, and 19 days post-wounding, spanning the inflammatory, proliferative, and remodeling phases of skin repair. We extracted nuclei with a two-step tissue dissociation protocol and prepared libraries using the DNBelab C Series Single-Nucleus ATAC Kit, then sequenced on the MGI DNBSEQ-T1 platform. After stringent quality filtering, 37,555 nuclei passed quality control; the resulting chromatin accessibility profiles cover 23 cell types: fibroblast subpopulations, keratinocytes, vascular endothelial cells, immune cells, and skin appendage cell types. This genome-wide chromatin accessibility dataset is a resource for studying regulatory dynamics during excisional wound repair and for comparing results with other wound healing systems.
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BibTeX
@misc{chai2026singlenucleus,
title = {{Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin}},
author = {C. Chai and X. Ding and Y. Li and L. Liang and R. Huang and W. Zhang and R. Li and S. Xie and X. Zeng and L. Zhang and Y. Yuan and X. Wei and J. Wang and S. Wang and S. Ji},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.15.759293},
url = {https://www.biorxiv.org/content/10.64898/2026.10.15.759293v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Single-nucleus chromatin accessibility time course of excisional wound repair in rat skin
AU - C. Chai
AU - X. Ding
AU - Y. Li
AU - L. Liang
AU - R. Huang
AU - W. Zhang
AU - R. Li
AU - S. Xie
AU - X. Zeng
AU - L. Zhang
AU - Y. Yuan
AU - X. Wei
AU - J. Wang
AU - S. Wang
AU - S. Ji
PY - 2026
DA - 2026/10/15
PB - bioRxiv
DO - 10.64898/2026.10.15.759293
UR - https://www.biorxiv.org/content/10.64898/2026.10.15.759293v1
AB - We present a time-series single-nucleus ATAC-seq dataset from full-thickness excisional wounds in Sprague-Dawley rats. Samples were taken from unwounded skin (normal control) and at 1, 2, 5, 10, and 19 days post-wounding, spanning the inflammatory, proliferative, and remodeling phases of skin repair. We extracted nuclei with a two-step tissue dissociation protocol and prepared libraries using the DNBelab C Series Single-Nucleus ATAC Kit, then sequenced on the MGI DNBSEQ-T1 platform. After stringent quality filtering, 37,555 nuclei passed quality control; the resulting chromatin accessibility profiles cover 23 cell types: fibroblast subpopulations, keratinocytes, vascular endothelial cells, immune cells, and skin appendage cell types. This genome-wide chromatin accessibility dataset is a resource for studying regulatory dynamics during excisional wound repair and for comparing results with other wound healing systems.
ER -