Identification and Molecular Characterization of a TYLCV Novel Isolate Associated with Disease in Tomato Cultivars Carrying Bred Resistance
- 1College of Life Sciences, Shandong Agricultural University, Taian 271018, China
- 2Key Laboratory of Agricultural Biotechnology of Yunnan Province, Institute of Biotechnology and Germplasm Rescouces, Yunnan Academy of Agricultural Sciences, Kunming 650205, China
- 3Vector-Borne Virus Research Center, State Key Laboratory for Ecological Pest Control of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract
Tomato yellow leaf curl virus (TYLCV) is a devastating viral pathogen threatening agricultural crops globally. In this study, we identified a novel TYLCV isolate (TYLCV-YN6244), which was associated with disease outbreaks in resistant tomato cultivars in Yuanmou County in Yunnan Province, China. We determined the complete genome of TYLCV-YN6244 and found it encoded six viral proteins characteristic of Geminivirus. We identified its V2 protein as a potent viral suppressor of RNA silencing (VSR), and generated an infectious clone of wildtype TYLCV-YN6244, or V2-defective TYLCV-YN6244 (TYLCV-YN6244-ΔV2) in which V2 was deleted. Both infectious clones were capable of systemically infecting tobacco and tomato. However, TYLCV-YN6244 but not TYLCV-YN6244-ΔV2 could cause disease symptoms in wildtype tobacco or tomato plants, and viral accumulation was drastically reduced in plants infected with TYLCV-YN6244-ΔV2 compared to TYLCV-YN6244 while the efficiency of virus-derived small interfering RNAs (vsiRNAs) biogenesis was conversely increased in plants infected with TYLCV-YN6244-ΔV2. Surprisingly, small RNA profiling indicated that the 21nt and 22nt rather than 24nt vsiRNAs were predominantly produced in tomato plants infected with either TYLCV-YN6244 or TYLCV-YN6244-ΔV2. It is likely that 21nt and 22nt vsiRNAs play a significant role in plant resistance to DNA viruses. Furthermore, transcriptome analyses revealed that TYLCV-YN6244 or TYLCV-YN6244-ΔV2 infection differentially modulated metabolism and defense-related pathways in tomato, probably underlying distinct viral pathogenicity and disease symptoms induced in plants. Overall, our research not only identified a novel pathogenic TYLCV isolate but also characterized molecular biology and host response in tomato with infectious clones developed in this study, with implications for untangling virus–host interaction for developing novel resistance in crop tomato.
Related
- Molecular and genomic characterization of biofilm associated antimicrobial resistance in clinical and resistant isolates of Pseudomonas aeruginosa in Iraq
- Transcriptional signatures associated with environmental and developmental variation in tobacco aroma-related pathways across cultivars and regions
- Identification and characterization of two transmembrane proteins required for virulence of Ustilago maydis
- Identification and functional characterization of novel murine DIS3L2 isoforms
- Identification of a Conserved Temporal Molecular Response Signature to CDK4/6 Inhibition Through Multi-Omics Integration Across Cancer Cell Lines
BibTeX
@article{zhou2026identification,
title = {{Identification and Molecular Characterization of a TYLCV Novel Isolate Associated with Disease in Tomato Cultivars Carrying Bred Resistance}},
author = {Yaogui Zhou and Jing Zhong and Shihuai Jin and Xingming Xiao and Ming Ding and Liling Zhao and Zhongxin Guo},
journal = {Biomolecules},
year = {2026},
month = nov,
doi = {10.3390/biom16111627},
url = {https://www.mdpi.com/2218-273X/16/11/1627},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Identification and Molecular Characterization of a TYLCV Novel Isolate Associated with Disease in Tomato Cultivars Carrying Bred Resistance
AU - Yaogui Zhou
AU - Jing Zhong
AU - Shihuai Jin
AU - Xingming Xiao
AU - Ming Ding
AU - Liling Zhao
AU - Zhongxin Guo
PY - 2026
DA - 2026/11/08
JO - Biomolecules
DO - 10.3390/biom16111627
UR - https://www.mdpi.com/2218-273X/16/11/1627
AB - Tomato yellow leaf curl virus (TYLCV) is a devastating viral pathogen threatening agricultural crops globally. In this study, we identified a novel TYLCV isolate (TYLCV-YN6244), which was associated with disease outbreaks in resistant tomato cultivars in Yuanmou County in Yunnan Province, China. We determined the complete genome of TYLCV-YN6244 and found it encoded six viral proteins characteristic of Geminivirus. We identified its V2 protein as a potent viral suppressor of RNA silencing (VSR), and generated an infectious clone of wildtype TYLCV-YN6244, or V2-defective TYLCV-YN6244 (TYLCV-YN6244-ΔV2) in which V2 was deleted. Both infectious clones were capable of systemically infecting tobacco and tomato. However, TYLCV-YN6244 but not TYLCV-YN6244-ΔV2 could cause disease symptoms in wildtype tobacco or tomato plants, and viral accumulation was drastically reduced in plants infected with TYLCV-YN6244-ΔV2 compared to TYLCV-YN6244 while the efficiency of virus-derived small interfering RNAs (vsiRNAs) biogenesis was conversely increased in plants infected with TYLCV-YN6244-ΔV2. Surprisingly, small RNA profiling indicated that the 21nt and 22nt rather than 24nt vsiRNAs were predominantly produced in tomato plants infected with either TYLCV-YN6244 or TYLCV-YN6244-ΔV2. It is likely that 21nt and 22nt vsiRNAs play a significant role in plant resistance to DNA viruses. Furthermore, transcriptome analyses revealed that TYLCV-YN6244 or TYLCV-YN6244-ΔV2 infection differentially modulated metabolism and defense-related pathways in tomato, probably underlying distinct viral pathogenicity and disease symptoms induced in plants. Overall, our research not only identified a novel pathogenic TYLCV isolate but also characterized molecular biology and host response in tomato with infectious clones developed in this study, with implications for untangling virus–host interaction for developing novel resistance in crop tomato.
SN - 2218-273X
ER -