Characterization and Antibacterial Activity of Biosynthesized Zinc Oxide–Silver (ZnO–Ag) Nanocomposites Using Manihot esculenta Leaf Extract
- 1Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami, Surakarta, Central Java 57126, Indonesia
- 2Department of Life Sciences, Abasyn University, Islamabad Campus, Park Road, Islamabad 44000, Pakistan
Abstract
Pseudomonas aeruginosa, an important opportunistic pathogen responsible for nosocomial infections, poses a threat in clinical care. Metal-metal oxide nanocomposites have been developed as antibacterial agents due to their synergistic effects. In this study, zinc oxide-silver nanocomposites (ZnO–AgNCs) were synthesized using Manihot esculenta leaf extract as a bioreductant, with extract concentrations of 5%, 10%, and 15% (v/v) and AgNO₃ concentrations of 6 and 8 mM. The samples were characterized using UV-Vis spectrophotometry, PSA, SEM-EDX, and their antibacterial activity was tested using the disk diffusion method. The best formulation was obtained for ZnO–AgNCs with 5% extract + 8 mM AgNO₃, which exhibited an SPR peak at 331 nm and an average particle size of 48.04 nm. SEM-EDX revealed an irregular and agglomerated morphology and confirmed the presence of Zn, O, and Ag. The nanocomposite showed strong antibacterial activity against P. aeruginosa with an inhibition zone of 15.83±0.64 mm. These findings highlight its potential for further development in infection control, particularly in medical applications. This study supports the Sustainable Development Goal (SDGs) on Good Health and Well-being (No. 3) through the development of antibacterial materials.
Related
- Oxytetracycline loading onto Cu–CuO/Cu2O nanocomposites (NCs): structural characterization and enhanced antimicrobial activity
- Eco-friendly synthesis and multifunctional characterization of ZrO₂/Cu₄O₃/MnO₂/CuMnO₂ nanocomposite with enhanced thermal stability and antibacterial activity.
- Hydrothermal Synthesis of Water-Dispersible Fluorescent Carbon Dots from Renewable Plant Precursors: Antibacterial Activity and Comparative Characterization
- Surface-modified magnetic polymeric–silver nanocomposites for broad-spectrum antibacterial applications
- Silver-Bacterial Cellulose-Areca catechu Extract Nanocomposite Biofilm for Sustainable Food Packaging: Synthesis, Characterization, and Antibacterial Activity.
BibTeX
@article{hafizhah2026characterization,
title = {{Characterization and Antibacterial Activity of Biosynthesized Zinc Oxide–Silver (ZnO–Ag) Nanocomposites Using Manihot esculenta Leaf Extract}},
author = {Wardah Radhwa Hafizhah and Ari Susilowati and Artini Pangastuti and Muhammad Ali},
journal = {Biosaintifika: Journal of Biology \& Biology Education},
year = {2026},
month = aug,
doi = {10.15294/biosaintifika.v18i2.40659},
url = {https://doi.org/10.15294/biosaintifika.v18i2.40659},
note = {CC BY},
}
RIS
TY - JOUR
TI - Characterization and Antibacterial Activity of Biosynthesized Zinc Oxide–Silver (ZnO–Ag) Nanocomposites Using Manihot esculenta Leaf Extract
AU - Wardah Radhwa Hafizhah
AU - Ari Susilowati
AU - Artini Pangastuti
AU - Muhammad Ali
PY - 2026
DA - 2026/08/01
JO - Biosaintifika: Journal of Biology & Biology Education
DO - 10.15294/biosaintifika.v18i2.40659
UR - https://doi.org/10.15294/biosaintifika.v18i2.40659
AB - Pseudomonas aeruginosa, an important opportunistic pathogen responsible for nosocomial infections, poses a threat in clinical care. Metal-metal oxide nanocomposites have been developed as antibacterial agents due to their synergistic effects. In this study, zinc oxide-silver nanocomposites (ZnO–AgNCs) were synthesized using Manihot esculenta leaf extract as a bioreductant, with extract concentrations of 5%, 10%, and 15% (v/v) and AgNO₃ concentrations of 6 and 8 mM. The samples were characterized using UV-Vis spectrophotometry, PSA, SEM-EDX, and their antibacterial activity was tested using the disk diffusion method. The best formulation was obtained for ZnO–AgNCs with 5% extract + 8 mM AgNO₃, which exhibited an SPR peak at 331 nm and an average particle size of 48.04 nm. SEM-EDX revealed an irregular and agglomerated morphology and confirmed the presence of Zn, O, and Ag. The nanocomposite showed strong antibacterial activity against P. aeruginosa with an inhibition zone of 15.83±0.64 mm. These findings highlight its potential for further development in infection control, particularly in medical applications. This study supports the Sustainable Development Goal (SDGs) on Good Health and Well-being (No. 3) through the development of antibacterial materials.
SN - 2085-191X
SN - 2338-7610
ER -