Technical validation and feasibility of video particle tracking for low-volume measurement of biliary micro-viscosity after liver transplantation
Abstract
Reliable quantification of the biophysical properties of human bile requires analytically validated methods and standardized pre-analytical conditions. We hypothesized that biliary micro-viscosity can be quantified in human bile after liver transplantation (LTx) with predefined analytical performance (accuracy ≤ 15% deviation from reference, reproducibility CV <10% ). In this method-development and technical-validation study, we established a method to quantify micro-viscosity in human bile after LTx. Validation using sucrose standards demonstrated high accuracy ( ≤ 15% deviation from reference), strong linear correlation ( R2 = 0.996 ), and good reproducibility (CV <10% ). The method was applied to 21 bile samples from nine patients collected longitudinally after LTx. Mean micro-viscosity was 1.58 ± 0.54 mPa s (postoperative day 1), 1.94 ± 0.63 mPa s (day 7), and 1.44 ± 0.16 mPa s (day 14). Pre-analytical freezing and cooling introduced measurement variability, supporting analysis shortly after collection. These findings demonstrate the analytical performance and practical feasibility of video particle-tracking-based biliary micro-viscosity measurements after LTx. Larger studies combining standardized fresh-sample workflows, biochemical bile characterization, and longitudinal clinical outcome data are required to determine the biological relevance of biliary micro-viscosity and its potential value for future graft modeling.
Related
- Technical feasibility of synchronized multispectral dual-site optical sensing during vascular occlusion in an acute porcine flap model
- Transcatheter Valve-in-Band Implantation in a Swine Model of Failed Mitral Repair: A Technical Note and Proof of Feasibility
- Fundamental Strategies and Technical Perspectives in Bile Duct Anastomosis for Living Donor Liver Transplantation: Growth From Novice to Maturity
- Deep-Learning-Based Assessment of Breast Arterial Calcification on Mammography: Technical Validation, Cardiovascular Prognostic Value, and Clinical Translation—A Narrative Review
- French validation of the short version of the ASA questionnaire: An exploration of the socio-technical imaginaries and cosmology of Socially Interactive Agent evaluation
BibTeX
@article{lenz2026technical,
title = {{Technical validation and feasibility of video particle tracking for low-volume measurement of biliary micro-viscosity after liver transplantation}},
author = {Ludwig Lenz and Philip Friedrich and Peggy Stock and Sandra Nickel and Luis Mandl and Anton Schnurpel and Fabian Haak and Uwe Scheuermann and Rebecca Sternkopf and Tim Ricken and Josef Alfons Käs and Hans-Michael Tautenhahn},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-026-74034-0},
url = {https://www.nature.com/articles/s41598-026-74034-0},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Technical validation and feasibility of video particle tracking for low-volume measurement of biliary micro-viscosity after liver transplantation
AU - Ludwig Lenz
AU - Philip Friedrich
AU - Peggy Stock
AU - Sandra Nickel
AU - Luis Mandl
AU - Anton Schnurpel
AU - Fabian Haak
AU - Uwe Scheuermann
AU - Rebecca Sternkopf
AU - Tim Ricken
AU - Josef Alfons Käs
AU - Hans-Michael Tautenhahn
PY - 2026
DA - 2026/10/10
JO - Scientific Reports
DO - 10.1038/s41598-026-74034-0
UR - https://www.nature.com/articles/s41598-026-74034-0
AB - Reliable quantification of the biophysical properties of human bile requires analytically validated methods and standardized pre-analytical conditions. We hypothesized that biliary micro-viscosity can be quantified in human bile after liver transplantation (LTx) with predefined analytical performance (accuracy ≤ 15% deviation from reference, reproducibility CV <10% ). In this method-development and technical-validation study, we established a method to quantify micro-viscosity in human bile after LTx. Validation using sucrose standards demonstrated high accuracy ( ≤ 15% deviation from reference), strong linear correlation ( R^2 = 0.996 ), and good reproducibility (CV <10% ). The method was applied to 21 bile samples from nine patients collected longitudinally after LTx. Mean micro-viscosity was 1.58 ± 0.54 mPa s (postoperative day 1), 1.94 ± 0.63 mPa s (day 7), and 1.44 ± 0.16 mPa s (day 14). Pre-analytical freezing and cooling introduced measurement variability, supporting analysis shortly after collection. These findings demonstrate the analytical performance and practical feasibility of video particle-tracking-based biliary micro-viscosity measurements after LTx. Larger studies combining standardized fresh-sample workflows, biochemical bile characterization, and longitudinal clinical outcome data are required to determine the biological relevance of biliary micro-viscosity and its potential value for future graft modeling.
SN - 2045-2322
ER -