Interaction characteristics of RAW264.7 macrophages with
- 1Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai’an, 271017, China; Shandong Provincial Key Laboratory of Zoonoses, Shandong Agricultural University, Tai’an, 271017, China
Abstract
Streptococcus suis serotype 2 (SS2) is a highly pathogenic zoonotic agent responsible for severe systemic infections in both humans and pigs. Its pathogenesis is closely associated with host immune dysregulation, which is further aggravated by high bacterial load in the infected host. We hypothesized that the multiplicity of infection (MOI) plays an important role in SS2 evasion of macrophage-mediated immunity by modulating macrophage function. RAW264.7 cells were infected with the SS2 strain ZY05719 at gradient MOIs, and their defensive capacity against SS2 was systematically assessed. At low MOIs, RAW264.7 cells efficiently phagocytosed SS2 and exhibited potent bactericidal activity. In contrast, when the MOI reached 20 or higher, SS2 markedly impaired the phagocytic and bactericidal capacities of RAW264.7 cells, inhibited ROS production and pro-inflammatory cytokine transcription, and even induced apoptosis, ultimately promoting the intracellular survival of SS2. Taken together, this study provides a novel insight into the role of MOI as a critical determinant in the interplay between SS2 and macrophages.
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BibTeX
@article{cheng2026interaction,
title = {{Interaction characteristics of RAW264.7 macrophages with}},
author = {Ying Cheng and Xinran Li and Shiqian Dang and Ruihua Zhang and Shijin Jiang and Yu Meng},
journal = {The Microbe},
year = {2026},
month = oct,
doi = {10.1016/j.microb.2026.100819},
url = {https://doi.org/10.1016/j.microb.2026.100819},
note = {CC BY},
}
RIS
TY - JOUR
TI - Interaction characteristics of RAW264.7 macrophages with
AU - Ying Cheng
AU - Xinran Li
AU - Shiqian Dang
AU - Ruihua Zhang
AU - Shijin Jiang
AU - Yu Meng
PY - 2026
DA - 2026/10/30
JO - The Microbe
DO - 10.1016/j.microb.2026.100819
UR - https://doi.org/10.1016/j.microb.2026.100819
AB - Streptococcus suis serotype 2 (SS2) is a highly pathogenic zoonotic agent responsible for severe systemic infections in both humans and pigs. Its pathogenesis is closely associated with host immune dysregulation, which is further aggravated by high bacterial load in the infected host. We hypothesized that the multiplicity of infection (MOI) plays an important role in SS2 evasion of macrophage-mediated immunity by modulating macrophage function. RAW264.7 cells were infected with the SS2 strain ZY05719 at gradient MOIs, and their defensive capacity against SS2 was systematically assessed. At low MOIs, RAW264.7 cells efficiently phagocytosed SS2 and exhibited potent bactericidal activity. In contrast, when the MOI reached 20 or higher, SS2 markedly impaired the phagocytic and bactericidal capacities of RAW264.7 cells, inhibited ROS production and pro-inflammatory cytokine transcription, and even induced apoptosis, ultimately promoting the intracellular survival of SS2. Taken together, this study provides a novel insight into the role of MOI as a critical determinant in the interplay between SS2 and macrophages.
SN - 2950-1946
ER -