Effects of Rumen-Protected β-Alanine on Hepatic Gluconeogenesis-Related Indices and Muscle Energy Metabolism in Transported Cattle.
- 1Jiangxi Province Key Laboratory of Animal Nutrition, Animal Nutrition and Feed Safety Innovation Team, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
- 2College of Life Science, Nanchang Normal University, Nanchang 330032, China.
Abstract
Preslaughter nutritional strategies that preserve energy reserves in transported beef cattle may help maintain normal post-mortem muscle metabolism and meat quality. β-alanine, a carnosine precursor, enhances glucose metabolism and glycogen reserves. This study evaluated the effects of rumen-protected β-alanine (RP-β-Ala) on meat quality, hepatic gluconeogenesis-related indices, and muscle energy metabolism in long-distance transported cattle. Thirty-six healthy 18-month-old Simmental crossbred bulls (627 ± 41 kg) were randomly assigned diets containing 0 (control) or 96 g/head/d RP-β-Ala. After 28 days of feeding, serum was sampled from six bulls per group. Following a 2-day rest (RP-β-Ala supplementation continued), these twelve bulls were transported, slaughtered, and sampled. Transport significantly increased serum lactate dehydrogenase (LDH) and creatine kinase (CK). The transport × RP-β-Ala interaction significantly affected serum superoxide dismutase (SOD). Post-slaughter tissue indices were analyzed solely for the effect of RP-β-Ala supplementation, as all slaughtered bulls underwent transportation. Compared to controls, the RP-β-Ala group exhibited significantly higher muscle pH45 min and an accelerated pH decline rate within 24 h post-mortem in both M. longissimus dorsi (LD) and M. semitendinosus (ST) muscles. In LD muscle, hardness, shear force, gumminess, and chewiness significantly decreased, while ATP content and HK, PFK, PK, and LDH activities significantly increased. In ST muscle, lactate content and GP, HK, PFK, and LDH activities significantly increased. In the liver, glucose, lactate, ATP, and ADP contents significantly increased, alongside enhanced G-6-Pase and pyruvate carboxylase (PC) activities. Dietary RP-β-Ala supplementation may regulate energy metabolism in transported cattle by enhancing muscle glycolytic activity and indices associated with hepatic gluconeogenic capacity, thereby improving selected meat-quality traits.
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BibTeX
@article{fu2026effects,
title = {{Effects of Rumen-Protected β-Alanine on Hepatic Gluconeogenesis-Related Indices and Muscle Energy Metabolism in Transported Cattle.}},
author = {Boya Fu and Yitian Zang and Haoxiang Xu and Daci Fu and Guwei Lu and Kang Mao and Qinghua Qiu and Xianghui Zhao and Mingren Qu and Kehui Ouyang and Yanjiao Li},
journal = {Animals : an open access journal from MDPI},
year = {2026},
month = sep,
doi = {10.3390/ani16193074},
url = {https://europepmc.org/article/MED/42883042},
note = {CC BY},
}
RIS
TY - JOUR
TI - Effects of Rumen-Protected β-Alanine on Hepatic Gluconeogenesis-Related Indices and Muscle Energy Metabolism in Transported Cattle.
AU - Boya Fu
AU - Yitian Zang
AU - Haoxiang Xu
AU - Daci Fu
AU - Guwei Lu
AU - Kang Mao
AU - Qinghua Qiu
AU - Xianghui Zhao
AU - Mingren Qu
AU - Kehui Ouyang
AU - Yanjiao Li
PY - 2026
DA - 2026/09/29
JO - Animals : an open access journal from MDPI
DO - 10.3390/ani16193074
UR - https://europepmc.org/article/MED/42883042
AB - Preslaughter nutritional strategies that preserve energy reserves in transported beef cattle may help maintain normal post-mortem muscle metabolism and meat quality. β-alanine, a carnosine precursor, enhances glucose metabolism and glycogen reserves. This study evaluated the effects of rumen-protected β-alanine (RP-β-Ala) on meat quality, hepatic gluconeogenesis-related indices, and muscle energy metabolism in long-distance transported cattle. Thirty-six healthy 18-month-old Simmental crossbred bulls (627 ± 41 kg) were randomly assigned diets containing 0 (control) or 96 g/head/d RP-β-Ala. After 28 days of feeding, serum was sampled from six bulls per group. Following a 2-day rest (RP-β-Ala supplementation continued), these twelve bulls were transported, slaughtered, and sampled. Transport significantly increased serum lactate dehydrogenase (LDH) and creatine kinase (CK). The transport × RP-β-Ala interaction significantly affected serum superoxide dismutase (SOD). Post-slaughter tissue indices were analyzed solely for the effect of RP-β-Ala supplementation, as all slaughtered bulls underwent transportation. Compared to controls, the RP-β-Ala group exhibited significantly higher muscle pH45 min and an accelerated pH decline rate within 24 h post-mortem in both M. longissimus dorsi (LD) and M. semitendinosus (ST) muscles. In LD muscle, hardness, shear force, gumminess, and chewiness significantly decreased, while ATP content and HK, PFK, PK, and LDH activities significantly increased. In ST muscle, lactate content and GP, HK, PFK, and LDH activities significantly increased. In the liver, glucose, lactate, ATP, and ADP contents significantly increased, alongside enhanced G-6-Pase and pyruvate carboxylase (PC) activities. Dietary RP-β-Ala supplementation may regulate energy metabolism in transported cattle by enhancing muscle glycolytic activity and indices associated with hepatic gluconeogenic capacity, thereby improving selected meat-quality traits.
SN - 2076-2615
ER -