Simple, Fast, and Ultrasensitive Fluorescent Detection of Peroxymonosulfate in Chemical, Enzymatic, and Cellular Systems.
- 1Institute of Polymer and Dye Technology, Faculty of Chemistry, Lodz University of Technology, Stefanowskiego 16, 90-537Lodz, Poland.
- 2Institute of Applied Radiation Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924Lodz, Poland.
- 3Department of Biophysics and Redox & Bioenergetics Core, Cancer Center Translational Metabolomics Shared Resource, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin53226, United States.
Abstract
Peroxymonosulfate (PMS) is widely used in advanced oxidation processes (AOP) to remove organic contaminants from wastewater. A simple method for determining PMS is necessary to dose this oxidant effectively and use it adequately in AOP. Here, we report a simple, fast, and accurate method for quantifying PMS using a pro-fluorescent boronate redox probe. In contrast to previously described methods, the proposed assay does not require activation of PMS by a low-valent transition metal. Based on the determined second-order rate constant of the direct oxidation reaction of the coumarin-7-boronic acid probe with PMS (2k = 1.1 × 104 M-1 s-1), we have developed a method based on high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) for ultrasensitive detection of PMS with a detection limit of 5 nM and a quantification limit of 40 nM. The HPLC-FLD method enables detection of PMS in real water samples, confirming its practical usefulness. The reported method enables the determination of PMS over a wide pH range and in the presence of common ions. It also enables tracking of PMS formation in sulfite solutions. Using activated RAW 264.7 macrophages, we demonstrate the feasibility of real-time monitoring of PMS production using a plate reader with fluorescence detection, along with appropriate control experiments.
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BibTeX
@article{podsiadly2026simple,
title = {{Simple, Fast, and Ultrasensitive Fluorescent Detection of Peroxymonosulfate in Chemical, Enzymatic, and Cellular Systems.}},
author = {Radosław Podsiadły and Aleksandra Grzelakowska and Kumari Neelam and Radosław Michalski and Michał Rostkowski and Renata Smulik-Izydorczyk and Adam Sikora and Jacek Zielonka},
journal = {Analytical chemistry},
year = {2026},
month = oct,
doi = {10.1021/acs.analchem.6c03097},
url = {https://europepmc.org/article/MED/42899527},
note = {CC BY},
}
RIS
TY - JOUR
TI - Simple, Fast, and Ultrasensitive Fluorescent Detection of Peroxymonosulfate in Chemical, Enzymatic, and Cellular Systems.
AU - Radosław Podsiadły
AU - Aleksandra Grzelakowska
AU - Kumari Neelam
AU - Radosław Michalski
AU - Michał Rostkowski
AU - Renata Smulik-Izydorczyk
AU - Adam Sikora
AU - Jacek Zielonka
PY - 2026
DA - 2026/10/01
JO - Analytical chemistry
DO - 10.1021/acs.analchem.6c03097
UR - https://europepmc.org/article/MED/42899527
AB - Peroxymonosulfate (PMS) is widely used in advanced oxidation processes (AOP) to remove organic contaminants from wastewater. A simple method for determining PMS is necessary to dose this oxidant effectively and use it adequately in AOP. Here, we report a simple, fast, and accurate method for quantifying PMS using a pro-fluorescent boronate redox probe. In contrast to previously described methods, the proposed assay does not require activation of PMS by a low-valent transition metal. Based on the determined second-order rate constant of the direct oxidation reaction of the coumarin-7-boronic acid probe with PMS (2k = 1.1 × 104 M-1 s-1), we have developed a method based on high-performance liquid chromatography coupled with fluorescence detection (HPLC-FLD) for ultrasensitive detection of PMS with a detection limit of 5 nM and a quantification limit of 40 nM. The HPLC-FLD method enables detection of PMS in real water samples, confirming its practical usefulness. The reported method enables the determination of PMS over a wide pH range and in the presence of common ions. It also enables tracking of PMS formation in sulfite solutions. Using activated RAW 264.7 macrophages, we demonstrate the feasibility of real-time monitoring of PMS production using a plate reader with fluorescence detection, along with appropriate control experiments.
SN - 0003-2700
SN - 1520-6882
ER -