Comprehensive Immune Repertoires of T Cell Receptor and B Cell Receptor Identify Key Immune Features Associated With Disease Activity of Systemic Lupus Erythematosus.
8 affiliations
- 1Department of Rheumatology and Immunology Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai China.
- 2Department of Paediatrics Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai China.
- 3Department of Critical Care Medicine Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai China.
- 4Department of Infectious Diseases Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai China.
- 5Department of Oncology Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai China.
- 6LeaLing Biopharma Co., Ltd. Suzhou China.
- 7Department of Rheumatology and Immunology The Fourth Affiliated Hospital of Xinjiang Medical University Xinjiang China.
- 8Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai China.
Abstract
We integrated immune repertoire sequencing (CD4+ and CD8+ T cell receptor [TCR], B cell receptor [BCR]) and transcriptional profiling to characterize the peripheral immune landscape in 32 active systemic lupus erythematosus (SLE) patients, 30 stable SLE patients, and 35 healthy donors. CD4+ and CD8+ TCR α- and β-chain repertoires in SLE patients showed lower diversity, reduced similarity, and more heterogeneous V-J usage, with CD8+ TCR profiles exhibiting relatively consistent stepwise changes across healthy, stable, and active groups. CD8+ T-cell transcriptomics revealed activity-associated upregulation of CD38, S100A11, IFITM3, and LAG3. In addition, BCR immunoglobulin heavy chain (IGH) repertoire analysis showed isotype-specific remodeling in SLE, characterized by contracted IgM/IgD repertoires, increased IgA isotype ratio and richness, and enhanced IgG diversity. Active SLE showed further increased IgG richness with rare-clone enrichment and higher IgM diversity. Among the TCR, BCR, and CD8+ T-cell transcriptomic features evaluated, IGH-derived metrics, particularly IgG- and IgA-associated features, showed apparent within-cohort discrimination between active and stable SLE. Genetic algorithm-based label-guided feature selection followed by hierarchical clustering revealed a multi-omics pattern associated with disease activity within the current cohort. Our study characterized peripheral immune repertoire and transcriptional alterations in SLE and highlighted their value for characterizing disease activity.
Related
- PhIP-Seq reveals geography- and disease-associated human herpesvirus antibody repertoires and distinct EBV response modules
- T Cell Plasticity in Systemic Lupus Erythematosus Revealed by Large‐Scale T Cell Receptor Repertoire and Transcriptome Studies
- Development of a comprehensive database of markers of ultra-processing to identify ultra-processed food in the UK according to the Nova classification
- Systemic lupus erythematosus is associated with age-related macular degeneration risk in a nationwide Korean cohort
- Cancer patient outcomes correlate with activating-KIR/ligand repertoires associated with distinct NK cell transcriptomic profiles
BibTeX
@article{wu2026comprehensive,
title = {{Comprehensive Immune Repertoires of T Cell Receptor and B Cell Receptor Identify Key Immune Features Associated With Disease Activity of Systemic Lupus Erythematosus.}},
author = {Jingyi Wu and Zhuochao Zhou and Anrui Yu and Xia Lin and Yuyang Ren and Jialin Teng and Dehao Zhu and Fan Wang and Yijun You and Xiangjun Ji and Puyu Wang and Hongpu Li and Chengde Yang and Wenbo Wang and Yawei Du and Junna Ye},
journal = {MedComm},
year = {2026},
month = oct,
doi = {10.1002/mco2.71087},
url = {https://europepmc.org/article/MED/42921308},
note = {CC BY},
}
RIS
TY - JOUR
TI - Comprehensive Immune Repertoires of T Cell Receptor and B Cell Receptor Identify Key Immune Features Associated With Disease Activity of Systemic Lupus Erythematosus.
AU - Jingyi Wu
AU - Zhuochao Zhou
AU - Anrui Yu
AU - Xia Lin
AU - Yuyang Ren
AU - Jialin Teng
AU - Dehao Zhu
AU - Fan Wang
AU - Yijun You
AU - Xiangjun Ji
AU - Puyu Wang
AU - Hongpu Li
AU - Chengde Yang
AU - Wenbo Wang
AU - Yawei Du
AU - Junna Ye
PY - 2026
DA - 2026/10/23
JO - MedComm
DO - 10.1002/mco2.71087
UR - https://europepmc.org/article/MED/42921308
AB - We integrated immune repertoire sequencing (CD4+ and CD8+ T cell receptor [TCR], B cell receptor [BCR]) and transcriptional profiling to characterize the peripheral immune landscape in 32 active systemic lupus erythematosus (SLE) patients, 30 stable SLE patients, and 35 healthy donors. CD4+ and CD8+ TCR α- and β-chain repertoires in SLE patients showed lower diversity, reduced similarity, and more heterogeneous V-J usage, with CD8+ TCR profiles exhibiting relatively consistent stepwise changes across healthy, stable, and active groups. CD8+ T-cell transcriptomics revealed activity-associated upregulation of CD38, S100A11, IFITM3, and LAG3. In addition, BCR immunoglobulin heavy chain (IGH) repertoire analysis showed isotype-specific remodeling in SLE, characterized by contracted IgM/IgD repertoires, increased IgA isotype ratio and richness, and enhanced IgG diversity. Active SLE showed further increased IgG richness with rare-clone enrichment and higher IgM diversity. Among the TCR, BCR, and CD8+ T-cell transcriptomic features evaluated, IGH-derived metrics, particularly IgG- and IgA-associated features, showed apparent within-cohort discrimination between active and stable SLE. Genetic algorithm-based label-guided feature selection followed by hierarchical clustering revealed a multi-omics pattern associated with disease activity within the current cohort. Our study characterized peripheral immune repertoire and transcriptional alterations in SLE and highlighted their value for characterizing disease activity.
SN - 2688-2663
ER -