Auxin-mediated RcARF8-RcWRKY29-RcERF98 module protects the rose petals against the Botrytis cinerea by…

Abstract

As one of the world’s most important ornamental crops, rose (Rosa sp.) is highly susceptible to grey mold disease caused by Botrytis cinerea. Outbreaks of this disease during long-distance transportation often lead to substantial economic losses. This study identifies the WRKY transcription factor RcWRKY29 as a key regulator in the defense response against B. cinerea. Integrated RNA sequencing and DNA affinity purification sequencing analyses revealed RcERF98 as a direct target of RcWRKY29. Knockdown or overexpression RcWRKY29 correspondingly altered RcERF98 transcript levels, which in turn modulated ascorbic acid (AsA) accumulation and reactive oxygen species (ROS) scavenging in petals. Furthermore, exogenous application of naphthalene acetic acid significantly enhanced disease resistance and concurrently upregulated RcWRKY29 expression. We demonstrated that the auxin response factor RcARF8, but not RcARF1, binds to the RcWRKY29 promoter and activates its transcription. Moreover, knocking down or overexpression of RcARF8 affected the expression of both RcWRKY29 and RcERF98, thereby regulating AsA biosynthesis and ROS homeostasis, and ultimately determining disease susceptibility. Collectively, our findings reveal a signaling module in which the auxin-mediated transcription cascade involving RcARF8-RcWRKY29-RcERF98 confers resistance to B. cinerea by coordinating AsA-dependent ROS scavenging in rose petals.

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BibTeX

@article{wu2026auxinmediated,
  title = {{Auxin-mediated RcARF8-RcWRKY29-RcERF98 module protects the rose petals against the Botrytis cinerea by modulating ascorbic acid biosynthesis and reactive oxygen species scavenging}},
  author = {Jie Wu and Mengyue Jing and Shiqiu Yang and Qiushi Li and Daliang Li and Qi Fu and Xujun Ma and Dan Wang and Jiaxing Li and Hongying Jian and Huijun Yan and Kaixue Tang and Hao Zhang and Xianqin Qiu and Weikun Jing},
  journal = {Horticulture research},
  year = {2026},
  month = jun,
  doi = {10.1093/hr/uhag224},
  url = {https://europepmc.org/article/PMC/PMC13722868},
  note = {CC BY},
}

RIS

TY  - JOUR
TI  - Auxin-mediated RcARF8-RcWRKY29-RcERF98 module protects the rose petals against the Botrytis cinerea by modulating ascorbic acid biosynthesis and reactive oxygen species scavenging
AU  - Jie Wu
AU  - Mengyue Jing
AU  - Shiqiu Yang
AU  - Qiushi Li
AU  - Daliang Li
AU  - Qi Fu
AU  - Xujun Ma
AU  - Dan Wang
AU  - Jiaxing Li
AU  - Hongying Jian
AU  - Huijun Yan
AU  - Kaixue Tang
AU  - Hao Zhang
AU  - Xianqin Qiu
AU  - Weikun Jing
PY  - 2026
DA  - 2026/06/03
JO  - Horticulture research
DO  - 10.1093/hr/uhag224
UR  - https://europepmc.org/article/PMC/PMC13722868
AB  - As one of the world’s most important ornamental crops, rose (Rosa sp.) is highly susceptible to grey mold disease caused by Botrytis cinerea. Outbreaks of this disease during long-distance transportation often lead to substantial economic losses. This study identifies the WRKY transcription factor RcWRKY29 as a key regulator in the defense response against B. cinerea. Integrated RNA sequencing and DNA affinity purification sequencing analyses revealed RcERF98 as a direct target of RcWRKY29. Knockdown or overexpression RcWRKY29 correspondingly altered RcERF98 transcript levels, which in turn modulated ascorbic acid (AsA) accumulation and reactive oxygen species (ROS) scavenging in petals. Furthermore, exogenous application of naphthalene acetic acid significantly enhanced disease resistance and concurrently upregulated RcWRKY29 expression. We demonstrated that the auxin response factor RcARF8, but not RcARF1, binds to the RcWRKY29 promoter and activates its transcription. Moreover, knocking down or overexpression of RcARF8 affected the expression of both RcWRKY29 and RcERF98, thereby regulating AsA biosynthesis and ROS homeostasis, and ultimately determining disease susceptibility. Collectively, our findings reveal a signaling module in which the auxin-mediated transcription cascade involving RcARF8-RcWRKY29-RcERF98 confers resistance to B. cinerea by coordinating AsA-dependent ROS scavenging in rose petals.
SN  - 2662-6810
SN  - 2052-7276
ER  -

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