In Vitro Antimicrobial Activity of Chlorhexidine-Curcumin Nanoformulations for Teat Dipping Against Mastitis Pathogens.
5 affiliations
- 1Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
- 2Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand.
- 3Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai 50100, Thailand.
- 4Laboratory of Animal Production Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
- 5Asian Satellite Campuses Institute, Nagoya University, Overseas Campus, Nagoya 464-8601, Japan.
Abstract
Bovine mastitis is a major problem affecting dairy production, and teat disinfection is important for reducing the risk of bacterial infection. Curcumin has antimicrobial properties, but its poor water solubility limits its use in aqueous formulations. This study aimed to develop chlorhexidine-curcumin nanoformulations (CCNs) and perform preliminary in vitro screening of these nanoformulations as candidate teat-dipping formulations. Four CCNs containing increasing concentrations of curcumin (0%, 0.1%, 0.5%, and 0.9% w/v; CCN0, CCN0.1, CCN0.5, and CCN0.9, respectively) were prepared. CCN0, CCN0.1, and CCN0.5 were formulated as oil-in-water nanoemulsions, whereas CCN0.9 was prepared as a curcumin-β-cyclodextrin inclusion complex. The physicochemical properties were characterized, and the antibacterial activity was evaluated in vitro by agar well diffusion against six mastitis-associated pathogens. CCN0.9 showed the largest inhibition zones overall (20.07 ± 1.05-28.85 ± 1.05 mm; p < 0.05), while CCN0.1 and CCN0.5 showed activity comparable to or greater than that of commercial teat dips against several pathogens. CCN0.5 showed the most favorable nanoemulsion characteristics, with a particle size of 94.77 nm and a zeta potential of -46.57 mV. Overall, CCNs demonstrated antibacterial activity against mastitis pathogens, supporting their potential as candidate teat-dip formulations. Further teat irritation and in vivo efficacy studies are needed before field application.
Related
- SEVI fibrils are induced by bacterial surface molecules and exert antimicrobial activity against ESKAPE pathogens
- Editorial: Evolution of antimicrobial resistance in intracellular bacterial pathogens: a genomic and historical perspective
- LysM: a bacterial self-encoded peptidoglycan hydrolase as an antimicrobial and anti-biofilm agent for Gram-negative nosocomial pathogens
- Whole-Genome Sequencing Unravels Virulence and Antimicrobial Resistance Landscapes of Major Bovine Respiratory Disease Pathogens.
- Reptiles in the One Health ecology of antimicrobial resistance, with insights into zoonotic pathogens: reservoirs, transmission pathways, and public health implications
BibTeX
@article{chuasakhonwilai2026vitro,
title = {{In Vitro Antimicrobial Activity of Chlorhexidine-Curcumin Nanoformulations for Teat Dipping Against Mastitis Pathogens.}},
author = {Areerat Chuasakhonwilai and Wasana Chaisri and Montira Intanon and Duanghathai Saipinta and Patsorn Keeklangdon and Pimnipa Jieraviriyapun and Sho Nakamura and Satoshi Ohkura and Witaya Suriyasathaporn},
journal = {Veterinary sciences},
year = {2026},
month = oct,
doi = {10.3390/vetsci13101054},
url = {https://europepmc.org/article/MED/42944150},
note = {CC BY},
}
RIS
TY - JOUR
TI - In Vitro Antimicrobial Activity of Chlorhexidine-Curcumin Nanoformulations for Teat Dipping Against Mastitis Pathogens.
AU - Areerat Chuasakhonwilai
AU - Wasana Chaisri
AU - Montira Intanon
AU - Duanghathai Saipinta
AU - Patsorn Keeklangdon
AU - Pimnipa Jieraviriyapun
AU - Sho Nakamura
AU - Satoshi Ohkura
AU - Witaya Suriyasathaporn
PY - 2026
DA - 2026/10/08
JO - Veterinary sciences
DO - 10.3390/vetsci13101054
UR - https://europepmc.org/article/MED/42944150
AB - Bovine mastitis is a major problem affecting dairy production, and teat disinfection is important for reducing the risk of bacterial infection. Curcumin has antimicrobial properties, but its poor water solubility limits its use in aqueous formulations. This study aimed to develop chlorhexidine-curcumin nanoformulations (CCNs) and perform preliminary in vitro screening of these nanoformulations as candidate teat-dipping formulations. Four CCNs containing increasing concentrations of curcumin (0%, 0.1%, 0.5%, and 0.9% w/v; CCN0, CCN0.1, CCN0.5, and CCN0.9, respectively) were prepared. CCN0, CCN0.1, and CCN0.5 were formulated as oil-in-water nanoemulsions, whereas CCN0.9 was prepared as a curcumin-β-cyclodextrin inclusion complex. The physicochemical properties were characterized, and the antibacterial activity was evaluated in vitro by agar well diffusion against six mastitis-associated pathogens. CCN0.9 showed the largest inhibition zones overall (20.07 ± 1.05-28.85 ± 1.05 mm; p < 0.05), while CCN0.1 and CCN0.5 showed activity comparable to or greater than that of commercial teat dips against several pathogens. CCN0.5 showed the most favorable nanoemulsion characteristics, with a particle size of 94.77 nm and a zeta potential of -46.57 mV. Overall, CCNs demonstrated antibacterial activity against mastitis pathogens, supporting their potential as candidate teat-dip formulations. Further teat irritation and in vivo efficacy studies are needed before field application.
SN - 2306-7381
ER -