Bioprinted heterogenous multistructured biomaterial with localized functionalization for the repair of complex tissue
Abstract
The inherent complexity of cell arrangements and the diversity of tissues in the human body should be considered during the development of biomaterials The recent emergence of multi-head printing techniques opened the possibilities to create heterogeneous scaffolds to repair or replace parts of the human body. For instance, additive manufacturing could be used to produce heterogenous materials composed of soft and hard layers, mimicking the structure of the tooth, as in this case study. Today, the gold standard for tooth replacement poorly replicates its structure, and 3D printing can better mimic its structure, allowing cell recolonization and helping tissue repair.We used a homemade dual head extruder 3D printer to print two different inks made of silk polymers. The first one was combined with ceramic microparticles to create a shell, while the second one was a foam-like scaffold to form the soft core of the scaffold, recreating the core/shell architecture of a tooth. The materials demonstrated a good biocompatibility and bioactivity with fibroblasts and Dental Pulp Stem Cells which were able to proliferate within the structures. The functionalization of the foam with an antibacterial agent was successfully performed.This study shows that a multi-head 3D printer can be used to create complex tissues with a heterogenous structure, in this case a non-cellularized shell surrounding a foam core cellularized with pulp stem cells. It opens the way for alternatives to a full tissue replacement by creating a patient-specific, biomimetic implant.
Related
- Spatially heterogenous distributions of dipteran abundances identify priority areas for haemosporidian parasite surveillance
- A Reproducible MRI‐Based Pipeline for Longitudinal Tracking of Laser‐Assisted Bioprinted Cells in Three‐Dimensional Constructs
- German dairy cows expose heterogenous bovine meat and milk factor DNA repertoires in milk and reveal Rep expression in mammary tissue
- 3D-bioprinted skeletal muscle with tailored myofibre architecture
- Recent Advances in Immunomodulatory Biomaterial Strategies for Neural Tissue Healing
BibTeX
@article{lartigue2026bioprinted,
title = {{Bioprinted heterogenous multistructured biomaterial with localized functionalization for the repair of complex tissue}},
author = {Claire de Lartigue and Sabine Kuchler-Bopp and Aline Echalard and Naïma Ahmed Omar and Youri Arntz and Vincent Fitzpatrick and Florent Meyer and Christophe Egles},
journal = {Bioprinting},
year = {2026},
month = oct,
doi = {10.1016/j.bprint.2026.e00477},
url = {https://hal.science/hal-05797570v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - Bioprinted heterogenous multistructured biomaterial with localized functionalization for the repair of complex tissue
AU - Claire de Lartigue
AU - Sabine Kuchler-Bopp
AU - Aline Echalard
AU - Naïma Ahmed Omar
AU - Youri Arntz
AU - Vincent Fitzpatrick
AU - Florent Meyer
AU - Christophe Egles
PY - 2026
DA - 2026/10/13
JO - Bioprinting
DO - 10.1016/j.bprint.2026.e00477
UR - https://hal.science/hal-05797570v1
AB - The inherent complexity of cell arrangements and the diversity of tissues in the human body should be considered during the development of biomaterials The recent emergence of multi-head printing techniques opened the possibilities to create heterogeneous scaffolds to repair or replace parts of the human body. For instance, additive manufacturing could be used to produce heterogenous materials composed of soft and hard layers, mimicking the structure of the tooth, as in this case study. Today, the gold standard for tooth replacement poorly replicates its structure, and 3D printing can better mimic its structure, allowing cell recolonization and helping tissue repair.We used a homemade dual head extruder 3D printer to print two different inks made of silk polymers. The first one was combined with ceramic microparticles to create a shell, while the second one was a foam-like scaffold to form the soft core of the scaffold, recreating the core/shell architecture of a tooth. The materials demonstrated a good biocompatibility and bioactivity with fibroblasts and Dental Pulp Stem Cells which were able to proliferate within the structures. The functionalization of the foam with an antibacterial agent was successfully performed.This study shows that a multi-head 3D printer can be used to create complex tissues with a heterogenous structure, in this case a non-cellularized shell surrounding a foam core cellularized with pulp stem cells. It opens the way for alternatives to a full tissue replacement by creating a patient-specific, biomimetic implant.
SN - 2405-8866
ER -