Isolation and characterisation of Lytic Jumbo Klebsiella phage BMPCR_005026
- 1University of Leicester
Abstract
Background: Klebsiella pneumoniae is a key causative agent of multidrug resistant nosocomial infections. Bacteriophages offer a promising alternative treatment, but the diversity of Klebsiella species requires the characterisation of a broad range of phages, particularly the poorly characterised jumbo phages. Materials and Methods: Phage BMCPR_005026 was isolated from sewage against Klebsiella pneumoniae. Whole genome sequencing, transmission electron microscopy and host range analysis against a diverse set of 40 clinically relevant Klebsiella strains were used to characterise the phage. Results: The virion had a myovirus morphology and genomic analysis placed it within an undefined family in Caudoviricetes where it forms a new species within the genus Alcyoneusvirus. BMCPR_005026 could form plaques on 3 of the 40 Klebsiella strains tested (7.5%) and formed halos. Conclusion: BMCPR_005026 represents a new species of phage within the genus Alcyoneusvirus with activity against 3 Klebsiella KL-types.
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BibTeX
@misc{lloydevans2026isolation,
title = {{Isolation and characterisation of Lytic Jumbo Klebsiella phage BMPCR_005026}},
author = {I. Lloyd-Evans and M. Ford and J. Hazzard and C. Bartis and F. Patel and N. Roemer and K. Wright and K. Porter and M. Coleman and E. Galyov and S. Megremis and M. R. Clokie and A. D. Millard and S. Michniewski and R. C. Wilkinson},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.30.755614},
url = {https://www.biorxiv.org/content/10.64898/2026.09.30.755614v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Isolation and characterisation of Lytic Jumbo Klebsiella phage BMPCR_005026
AU - I. Lloyd-Evans
AU - M. Ford
AU - J. Hazzard
AU - C. Bartis
AU - F. Patel
AU - N. Roemer
AU - K. Wright
AU - K. Porter
AU - M. Coleman
AU - E. Galyov
AU - S. Megremis
AU - M. R. Clokie
AU - A. D. Millard
AU - S. Michniewski
AU - R. C. Wilkinson
PY - 2026
DA - 2026/09/30
PB - bioRxiv
DO - 10.64898/2026.09.30.755614
UR - https://www.biorxiv.org/content/10.64898/2026.09.30.755614v1
AB - Background: Klebsiella pneumoniae is a key causative agent of multidrug resistant nosocomial infections. Bacteriophages offer a promising alternative treatment, but the diversity of Klebsiella species requires the characterisation of a broad range of phages, particularly the poorly characterised jumbo phages. Materials and Methods: Phage BMCPR_005026 was isolated from sewage against Klebsiella pneumoniae. Whole genome sequencing, transmission electron microscopy and host range analysis against a diverse set of 40 clinically relevant Klebsiella strains were used to characterise the phage. Results: The virion had a myovirus morphology and genomic analysis placed it within an undefined family in Caudoviricetes where it forms a new species within the genus Alcyoneusvirus. BMCPR_005026 could form plaques on 3 of the 40 Klebsiella strains tested (7.5%) and formed halos. Conclusion: BMCPR_005026 represents a new species of phage within the genus Alcyoneusvirus with activity against 3 Klebsiella KL-types.
ER -