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Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

An improved protocol to isolate enriched immune cells from murine intestinal epithelium and lamina propria

  • Sydney B Crotts1
  • Virginia Smith Shapiro2
  1. 1Graduate School of Biomedical Science, Mayo Clinic, Rochester, MN, USA; Department of Immunology, Mayo Clinic, Rochester, MN, USA.
  2. 2Graduate School of Biomedical Science, Mayo Clinic, Rochester, MN, USA; Department of Immunology, Mayo Clinic, Rochester, MN, USA. Electronic address: [email protected].
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Abstract

The intestine is home to more immune cells than any other non-lymphatic peripheral tissue, but research of these cells is stymied by the technical difficulties involved in isolating them. Here, we describe a protocol to isolate highly viable immune cells from murine intestinal epithelium and lamina propria. This protocol can be used to characterize cells by flow cytometry or single-cell transcriptomics, as in Crotts et al., or by other analysis methods.

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Cite

BibTeXRIS
BibTeX
@article{crotts2026improved,
  title = {{An improved protocol to isolate enriched immune cells from murine intestinal epithelium and lamina propria}},
  author = {Sydney B Crotts and Virginia Smith Shapiro},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104864},
  url = {https://doi.org/10.1016/j.xpro.2026.104864},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - An improved protocol to isolate enriched immune cells from murine intestinal epithelium and lamina propria
AU  - Sydney B Crotts
AU  - Virginia Smith Shapiro
PY  - 2026
DA  - 2026/10/20
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104864
UR  - https://doi.org/10.1016/j.xpro.2026.104864
AB  - The intestine is home to more immune cells than any other non-lymphatic peripheral tissue, but research of these cells is stymied by the technical difficulties involved in isolating them. Here, we describe a protocol to isolate highly viable immune cells from murine intestinal epithelium and lamina propria. This protocol can be used to characterize cells by flow cytometry or single-cell transcriptomics, as in Crotts et al., or by other analysis methods.
SN  - 2666-1667
ER  -