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Peer-reviewedCC BYmicroPublication Biology

Draft genome assemblies of three Ligilactobacillus salivarius strains isolated from commercial layer hen litter

  • Benjamin Brehm-Stecher1
  • Ryan Donaldson1
  • Charlie Fisher1
  • William Jensen1
  • Alex Kottmeyer1
  • Paul Lueth2
  • Matthew Lutz1
  • Elizabeth Makovec1
  • Simon Alexander Radl1
  • Ainsley Wood1
  • Madison Stoltz1
  • Nick Peters1
1 more authors
  • Lucille C. Jonas3
  1. 1Department of Plant Pathology, Entomology and Microbiology, Iowa State University, Ames, IA, USA
  2. 2Immunobiology Graduate Program, Iowa State University, Ames, IA, USA
  3. 3Department of Animal Science, Iowa State University, Ames, IA, USA
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Abstract

Direct-fed probiotics in poultry can improve animal nutrition and health while also competing with human foodborne pathogens for colonization of the gastrointestinal tract. The objective of this study was to perform whole-genome sequencing on lactic acid bacteria isolated from commercial chicken litter to characterize genetic markers associated with probiotic potential. Three Ligilactobacillus salivarius strains were selected for whole-genome sequencing using Oxford Nanopore Technology. The genomes contained genes associated with sialic acid utilization, bacteriocin production, and antimicrobial resistance. While these markers may indicate suitability as probiotics in poultry, further experimentation is needed to verify the functionality of the identified genes.

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Cite

BibTeXRIS
BibTeX
@article{brehmstecher2026draft,
  title = {{Draft genome assemblies of three Ligilactobacillus salivarius strains isolated from commercial layer hen litter}},
  author = {Benjamin Brehm-Stecher and Ryan Donaldson and Charlie Fisher and William Jensen and Alex Kottmeyer and Paul Lueth and Matthew Lutz and Elizabeth Makovec and Simon Alexander Radl and Ainsley Wood and Madison Stoltz and Nick Peters and Lucille C. Jonas},
  journal = {microPublication Biology},
  year = {2026},
  month = sep,
  doi = {10.17912/micropub.biology.002411},
  url = {https://doi.org/10.17912/micropub.biology.002411},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Draft genome assemblies of three Ligilactobacillus salivarius strains isolated from commercial layer hen litter
AU  - Benjamin Brehm-Stecher
AU  - Ryan Donaldson
AU  - Charlie Fisher
AU  - William Jensen
AU  - Alex Kottmeyer
AU  - Paul Lueth
AU  - Matthew Lutz
AU  - Elizabeth Makovec
AU  - Simon Alexander Radl
AU  - Ainsley Wood
AU  - Madison Stoltz
AU  - Nick Peters
AU  - Lucille C. Jonas
PY  - 2026
DA  - 2026/09/01
JO  - microPublication Biology
DO  - 10.17912/micropub.biology.002411
UR  - https://doi.org/10.17912/micropub.biology.002411
AB  - Direct-fed probiotics in poultry can improve animal nutrition and health while also competing with human foodborne pathogens for colonization of the gastrointestinal tract. The objective of this study was to perform whole-genome sequencing on lactic acid bacteria isolated from commercial chicken litter to characterize genetic markers associated with probiotic potential. Three Ligilactobacillus salivarius strains were selected for whole-genome sequencing using Oxford Nanopore Technology. The genomes contained genes associated with sialic acid utilization, bacteriocin production, and antimicrobial resistance. While these markers may indicate suitability as probiotics in poultry, further experimentation is needed to verify the functionality of the identified genes.
SN  - 2578-9430
ER  -