BSA/LYZ-Mediated and Ultrasound-Assisted Synthesis of Silver Oxide Nanoparticles with Enhanced Bactericidal Performance
- 1School of Life Science and Technology, Shandong Second Medical University, Weifang, People’s Republic of China
- 2Department of Mechanical Engineering, Shandong Polytechnic University, Weifang, People’s Republic of China
Abstract
Introduction: Silver oxide nanoparticles (Ag2O NPs) have attracted considerable attention due to their excellent bactericidal performance; however, Ag2O NPs prepared by traditional chemical methods often suffer from issues such as large particle size and poor dispersibility, which limit their practical application.
Methods: In this study, a protein-mediated, ultrasound-assisted synthesis method was employed to prepare Ag2O NPs. By regulating the concentrations of bovine serum albumin (BSA) and lysozyme (LYZ), as well as their addition order under an ultrasonic field, a series of Ag2O NPs were synthesized and subjected to physicochemical characterization and bactericidal performance evaluation. Furthermore, the bactericidal mechanism of Ag2O NPs was investigated based on Ag⁺ release, bacterial morphology and Live/Dead staining.
Results: The results indicate that regulating the type and addition order of proteins could produce Ag2O NPs with different morphologies, among which those synthesized under ultrasonic conditions via BSA mediation exhibit the smallest hydrodynamic particle size (127.66 nm) and the highest absolute zeta potential (−37.20 mV), and show the highest bactericidal efficiencies against Escherichia coli and Staphylococcus aureus, reaching approximately 95.97% and 98.69%, respectively. However, at higher LYZ concentrations (1g/L), the bactericidal efficiency of both ultrasonic-mediated and post-ultrasonic-mediated Ag2O NPs decreases significantly. The bactericidal activity of the Ag2O NPs is mainly associated with the synergistic effect of Ag⁺ release and bacterial membrane damage.
Conclusion: This study provides a new strategy for the preparation of highly efficient bactericidal nanoparticles, and further analyzes their possible bactericidal mechanisms, providing useful insights for the design of novel antibacterial materials.
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BibTeX
@article{liu2026bsalyzmediated,
title = {{BSA/LYZ-Mediated and Ultrasound-Assisted Synthesis of Silver Oxide Nanoparticles with Enhanced Bactericidal Performance}},
author = {Rui Liu and Wenfang Li and Yuyiming Zuo and Fangchong Xing and Shujiao Zhang and Lidan Zhao},
journal = {International Journal of Nanomedicine},
year = {2026},
month = nov,
doi = {10.2147/ijn.s640128},
url = {https://doi.org/10.2147/ijn.s640128},
note = {CC BY-NC 4.0},
}
RIS
TY - JOUR
TI - BSA/LYZ-Mediated and Ultrasound-Assisted Synthesis of Silver Oxide Nanoparticles with Enhanced Bactericidal Performance
AU - Rui Liu
AU - Wenfang Li
AU - Yuyiming Zuo
AU - Fangchong Xing
AU - Shujiao Zhang
AU - Lidan Zhao
PY - 2026
DA - 2026/11/01
JO - International Journal of Nanomedicine
DO - 10.2147/ijn.s640128
UR - https://doi.org/10.2147/ijn.s640128
AB - Introduction: Silver oxide nanoparticles (Ag2O NPs) have attracted considerable attention due to their excellent bactericidal performance; however, Ag2O NPs prepared by traditional chemical methods often suffer from issues such as large particle size and poor dispersibility, which limit their practical application. Methods: In this study, a protein-mediated, ultrasound-assisted synthesis method was employed to prepare Ag2O NPs. By regulating the concentrations of bovine serum albumin (BSA) and lysozyme (LYZ), as well as their addition order under an ultrasonic field, a series of Ag2O NPs were synthesized and subjected to physicochemical characterization and bactericidal performance evaluation. Furthermore, the bactericidal mechanism of Ag2O NPs was investigated based on Ag⁺ release, bacterial morphology and Live/Dead staining. Results: The results indicate that regulating the type and addition order of proteins could produce Ag2O NPs with different morphologies, among which those synthesized under ultrasonic conditions via BSA mediation exhibit the smallest hydrodynamic particle size (127.66 nm) and the highest absolute zeta potential (−37.20 mV), and show the highest bactericidal efficiencies against Escherichia coli and Staphylococcus aureus, reaching approximately 95.97% and 98.69%, respectively. However, at higher LYZ concentrations (1g/L), the bactericidal efficiency of both ultrasonic-mediated and post-ultrasonic-mediated Ag2O NPs decreases significantly. The bactericidal activity of the Ag2O NPs is mainly associated with the synergistic effect of Ag⁺ release and bacterial membrane damage. Conclusion: This study provides a new strategy for the preparation of highly efficient bactericidal nanoparticles, and further analyzes their possible bactericidal mechanisms, providing useful insights for the design of novel antibacterial materials.
SN - 1176-9114
SN - 1178-2013
ER -