Peer-reviewedCC BY-NC-NDSTAR protocols
Protocol for scalable production of orally deliverable R-spondin1-loaded small extracellular vesicles for WNT-signaling activation and tissue regeneration.
4 affiliations
- 1The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong Province 510150, China; Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China. Electronic address: [email protected].
- 2Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.
- 3Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China; Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong Province 510080, China.
- 4The Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong Province 510150, China; Guangzhou National Laboratory, Guangzhou, Guangdong Province 510005, China. Electronic address: [email protected].
Abstract
R-spondin1 plays a pivotal role in potentiating canonical WNT/β-catenin signaling, a pathway critical for maintaining stem cell homeostasis and driving tissue regeneration. Here, we present a scalable, serum-free protocol for producing R-spondin1-loaded small extracellular vesicles (evRSPO1) from engineered HEK293 cells, with multimodal characterization of vesicle quality and bioactivity. We also describe steps for in vitro functional validation of the vesicles using mouse intestinal organoids and for in vivo therapeutic applications in mouse intestinal regeneration via oral delivery. For complete details on the use and execution of this protocol, please refer to Yang et al.¹,².
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BibTeX
@article{yang2026protocol,
title = {{Protocol for scalable production of orally deliverable R-spondin1-loaded small extracellular vesicles for WNT-signaling activation and tissue regeneration.}},
author = {Lingyan Yang and Ting Jiang and Shixiang Wang and Yue Yang and Zhiping Qiao and Yun-Shen Chan},
journal = {STAR protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2026.104891},
url = {https://europepmc.org/article/MED/42848513},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Protocol for scalable production of orally deliverable R-spondin1-loaded small extracellular vesicles for WNT-signaling activation and tissue regeneration.
AU - Lingyan Yang
AU - Ting Jiang
AU - Shixiang Wang
AU - Yue Yang
AU - Zhiping Qiao
AU - Yun-Shen Chan
PY - 2026
DA - 2026/10/08
JO - STAR protocols
DO - 10.1016/j.xpro.2026.104891
UR - https://europepmc.org/article/MED/42848513
AB - R-spondin1 plays a pivotal role in potentiating canonical WNT/β-catenin signaling, a pathway critical for maintaining stem cell homeostasis and driving tissue regeneration. Here, we present a scalable, serum-free protocol for producing R-spondin1-loaded small extracellular vesicles (evRSPO1) from engineered HEK293 cells, with multimodal characterization of vesicle quality and bioactivity. We also describe steps for in vitro functional validation of the vesicles using mouse intestinal organoids and for in vivo therapeutic applications in mouse intestinal regeneration via oral delivery. For complete details on the use and execution of this protocol, please refer to Yang et al.¹,².
SN - 2666-1667
ER -