Understanding Substrate Transport and Metabolism in Clostridium thermocellum to Improve Biofuel Production
- 1Dartmouth College
Abstract
As a cellulolytic specialist microbe, Clostridium thermocellum primarily ferments cellulose and its breakdown products. However, the full breadth of its substrate range remains poorly understood. In this work, we investigated the ability of C. thermocellum to transport and metabolize a diverse range of alternative carbon substrates. We performed tetrazolium dye assays to assess the ability of wild-type (WT) C. thermocellum (strain LL1004) to transport and metabolize 190 different substrates and identified 18 positive hits and 27 false positives. These findings guided the selection of 22 substrates for batch fermentations of WT on 10 g/L alternative substrate plus 1 g/L cellobiose. We identified eight substrates that were either fully or partially consumed (cellobiose, glucose, fructose, sorbitol, arbutin, turanose, pyruvate, glucose-6-phosphate), two that were interconverted (malate, fumarate), seven that may undergo conversion to unidentified fermentation products (xylose, sorbose, tagatose, galactose, mannose, rhamnose, fucose), and five that were not consumed (glycerol, acetate, lactate, propionate, xylitol). Sorbitol and pyruvate, which are more and less reduced than cellobiose, respectively, were selected for further analysis of their effects on redox balance and product formation. Batch fermentations on sorbitol and pyruvate demonstrated that ethanol production in C. thermocellum may be limited in part by the availability of reducing equivalents. Collectively, this work demonstrates the utility of alternative carbon substrates as a tool for perturbing metabolism, redox balance, and fermentation product distributions to further our understanding of the metabolism of anaerobic, thermophilic bacteria such as C. thermocellum for the production of biofuels and biochemicals of interest.
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BibTeX
@misc{richardson2026understanding,
title = {{Understanding Substrate Transport and Metabolism in Clostridium thermocellum to Improve Biofuel Production}},
author = {I. D. Richardson and B. D. Sharma and E. Y. Ma and R. S. Pilny and D. G. Olson},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.15.759513},
url = {https://www.biorxiv.org/content/10.64898/2026.10.15.759513v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Understanding Substrate Transport and Metabolism in Clostridium thermocellum to Improve Biofuel Production
AU - I. D. Richardson
AU - B. D. Sharma
AU - E. Y. Ma
AU - R. S. Pilny
AU - D. G. Olson
PY - 2026
DA - 2026/10/15
PB - bioRxiv
DO - 10.64898/2026.10.15.759513
UR - https://www.biorxiv.org/content/10.64898/2026.10.15.759513v1
AB - As a cellulolytic specialist microbe, Clostridium thermocellum primarily ferments cellulose and its breakdown products. However, the full breadth of its substrate range remains poorly understood. In this work, we investigated the ability of C. thermocellum to transport and metabolize a diverse range of alternative carbon substrates. We performed tetrazolium dye assays to assess the ability of wild-type (WT) C. thermocellum (strain LL1004) to transport and metabolize 190 different substrates and identified 18 positive hits and 27 false positives. These findings guided the selection of 22 substrates for batch fermentations of WT on 10 g/L alternative substrate plus 1 g/L cellobiose. We identified eight substrates that were either fully or partially consumed (cellobiose, glucose, fructose, sorbitol, arbutin, turanose, pyruvate, glucose-6-phosphate), two that were interconverted (malate, fumarate), seven that may undergo conversion to unidentified fermentation products (xylose, sorbose, tagatose, galactose, mannose, rhamnose, fucose), and five that were not consumed (glycerol, acetate, lactate, propionate, xylitol). Sorbitol and pyruvate, which are more and less reduced than cellobiose, respectively, were selected for further analysis of their effects on redox balance and product formation. Batch fermentations on sorbitol and pyruvate demonstrated that ethanol production in C. thermocellum may be limited in part by the availability of reducing equivalents. Collectively, this work demonstrates the utility of alternative carbon substrates as a tool for perturbing metabolism, redox balance, and fermentation product distributions to further our understanding of the metabolism of anaerobic, thermophilic bacteria such as C. thermocellum for the production of biofuels and biochemicals of interest.
ER -