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Peer-reviewedCC BYRevista da Sociedade Brasileira de Medicina Tropical

Molecular Detection of Arboviruses in Aedes spp. Larvae During Entomo-Virological Surveillance in a Hospital Area of Brasília, Federal District, Brazil.

  • Renato Lemos Pereira1,2
  • Kelliane Almeida de Medeiros1
  • Marcelo Banho de Andrade Reis3
  • Diane Sthefany Lima de Oliveira1
  • Mércia Tayane Pereira Dos Santos de Oliveira1,2
  • Laysa Michelle Rodrigues Santos1,2
  • Dalva Iara Ghilardi de Paula1,4
  • Angela Rosa André1
  • Marcelo Henrique Soller Ramada2,4
  • Wanderson Kleber de Oliveira1
4 affiliations
  1. 1Hospital das Forças Armadas, Direção Técnica de Ensino e Pesquisa, Divisão de Pesquisa, Brasília, DF. Brasil.
  2. 2Universidade Católica de Brasília, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Águas Claras, DF, Brasil.
  3. 3Secretaria de Educação do Distrito Federal, Brasília, DF, Brasil.
  4. 4Universidade Católica de Brasília, Programa de Pós-Graduação em Gerontologia, Águas Claras, DF, Brasil.
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Abstract

Background

Arboviruses in hospital environments may represent a neglected risk of nosocomial infections. This study investigated arboviruses in Aedes spp. larvae collected from a hospital area in Brasília, Federal District, Brazil.Methods

Eighteen ovitraps were deployed for 26 weeks. Hatched Aedes spp. larvae were pooled weekly, and viral detection was performed by reverse transcription-quantitative PCR.Results

Dengue virus serotypes 1, 2, and 4, Zika virus, and Chikungunya virus were identified, with an overall positivity rate of 30.76%.Conclusion

The surveillance strategy effectively detected arboviral RNA in a hospital setting, suggesting local arboviral circulation associated with the transmission triad.

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Cite

BibTeXRIS
BibTeX
@article{pereira2026molecular,
  title = {{Molecular Detection of Arboviruses in Aedes spp. Larvae During Entomo-Virological Surveillance in a Hospital Area of Brasília, Federal District, Brazil.}},
  author = {Renato Lemos Pereira and Kelliane Almeida de Medeiros and Marcelo Banho de Andrade Reis and Diane Sthefany Lima de Oliveira and Mércia Tayane Pereira Dos Santos de Oliveira and Laysa Michelle Rodrigues Santos and Dalva Iara Ghilardi de Paula and Angela Rosa André and Marcelo Henrique Soller Ramada and Wanderson Kleber de Oliveira},
  journal = {Revista da Sociedade Brasileira de Medicina Tropical},
  year = {2026},
  month = oct,
  doi = {10.1590/0037-8682-0105-2026},
  url = {https://europepmc.org/article/MED/42912082},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Molecular Detection of Arboviruses in Aedes spp. Larvae During Entomo-Virological Surveillance in a Hospital Area of Brasília, Federal District, Brazil.
AU  - Renato Lemos Pereira
AU  - Kelliane Almeida de Medeiros
AU  - Marcelo Banho de Andrade Reis
AU  - Diane Sthefany Lima de Oliveira
AU  - Mércia Tayane Pereira Dos Santos de Oliveira
AU  - Laysa Michelle Rodrigues Santos
AU  - Dalva Iara Ghilardi de Paula
AU  - Angela Rosa André
AU  - Marcelo Henrique Soller Ramada
AU  - Wanderson Kleber de Oliveira
PY  - 2026
DA  - 2026/10/16
JO  - Revista da Sociedade Brasileira de Medicina Tropical
DO  - 10.1590/0037-8682-0105-2026
UR  - https://europepmc.org/article/MED/42912082
AB  - Background  Arboviruses in hospital environments may represent a neglected risk of nosocomial infections. This study investigated arboviruses in Aedes spp. larvae collected from a hospital area in Brasília, Federal District, Brazil.Methods  Eighteen ovitraps were deployed for 26 weeks. Hatched Aedes spp. larvae were pooled weekly, and viral detection was performed by reverse transcription-quantitative PCR.Results  Dengue virus serotypes 1, 2, and 4, Zika virus, and Chikungunya virus were identified, with an overall positivity rate of 30.76%.Conclusion  The surveillance strategy effectively detected arboviral RNA in a hospital setting, suggesting local arboviral circulation associated with the transmission triad.
SN  - 0037-8682
SN  - 1678-9849
ER  -