Parallel Production of Uniform Artificial Multicellular Models Using Vibration-Induced Local Microvortices
- 1Graduate School of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, Japan, 112-8551
- 2Earth-Life Science Institute (ELSI, Institute of Science Tokyo, 2-12-1 Ookayama, Meguro-ku, Tokyo, Japan, 152-8550
Abstract
In this study, we propose a facile and efficient method for constructing artificial multicellular models via vibration-induced flow (VIF), a local fluid flow induced around vibrating microstructures. By applying circular vibration to a substrate with a cylindrical micropillar array, uniform micro-vortices were generated, into which giant unilamellar vesicles (GUVs), as artificial cells, rapidly accumulated at the center. We show that by modifying two GUV populations with complementary single-stranded DNAs, we can promote specific adhesion between GUVs, leading to uniform parallel aggregates. Furthermore, the pillar-array design controls the size of GUV aggregates. Finally, we embedded nanopore-forming α-hemolysin with Spytag and SpyCatcher system in GUV populations, allowing the lumens of assembled GUVs to channel with one another and enable close-proximity molecular communication. This technology will contribute to future research on fabricating artificial multicellular models with complex functions.
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BibTeX
@misc{miyashita2026parallel,
title = {{Parallel Production of Uniform Artificial Multicellular Models Using Vibration-Induced Local Microvortices}},
author = {Daiki Miyashita and Yui Katsumata and Xu Han and Zhitai Huang and Mamiko Tsugane and Tomoaki Matsuura and Hiroaki Suzuki},
howpublished = {ChemRxiv},
year = {2026},
month = oct,
doi = {10.26434/chemrxiv.15010019/v1},
url = {https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010019/v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Parallel Production of Uniform Artificial Multicellular Models Using Vibration-Induced Local Microvortices
AU - Daiki Miyashita
AU - Yui Katsumata
AU - Xu Han
AU - Zhitai Huang
AU - Mamiko Tsugane
AU - Tomoaki Matsuura
AU - Hiroaki Suzuki
PY - 2026
DA - 2026/10/07
PB - ChemRxiv
DO - 10.26434/chemrxiv.15010019/v1
UR - https://chemrxiv.org/doi/full/10.26434/chemrxiv.15010019/v1
AB - In this study, we propose a facile and efficient method for constructing artificial multicellular models via vibration-induced flow (VIF), a local fluid flow induced around vibrating microstructures. By applying circular vibration to a substrate with a cylindrical micropillar array, uniform micro-vortices were generated, into which giant unilamellar vesicles (GUVs), as artificial cells, rapidly accumulated at the center. We show that by modifying two GUV populations with complementary single-stranded DNAs, we can promote specific adhesion between GUVs, leading to uniform parallel aggregates. Furthermore, the pillar-array design controls the size of GUV aggregates. Finally, we embedded nanopore-forming α-hemolysin with Spytag and SpyCatcher system in GUV populations, allowing the lumens of assembled GUVs to channel with one another and enable close-proximity molecular communication. This technology will contribute to future research on fabricating artificial multicellular models with complex functions.
ER -