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Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

Spatiotemporal Analysis of Intranuclear Inclusion Body and Virus Formation in Human Adenovirus Type 5-Infected Cells

  • Hong Wu1
  • Yoshihiko Fujioka1
  • Shoichi Sakaguchi1
  • Chinami Odake1
  • Youichi Suzuki2
  • Takashi Nakano1
  1. 1Department of Microbiology and Infection Control, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-machi, Takatsuki 569-8686, Osaka, Japan
  2. 2Laboratory of Biosafety Research, Faculty of Medicine, Osaka Medical and Pharmaceutical University, 2-7 Daigaku-machi, Takatsuki 569-8686, Osaka, Japan
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Abstract

Human adenovirus type 5 (HAdV-5) forms a characteristic intranuclear inclusion body in infected cells. However, the relationship between the intranuclear inclusion body and virus formation in HAdV-5-infected cells remains unclear. This study conducted a detailed investigation of the relationship between the structure of the inclusion body and the localization of the virus in HAdV-5-infected cells using electron microscopy and three-dimensional (3D) tomography analytical techniques. Immunostaining for viral proteins was also performed to determine their colocalization with the intranuclear inclusion body. 3D analysis revealed the intranuclear inclusion body as the major site of viral particle accumulation in the cells. Viral particles were identified within the intranuclear inclusion body and arranged in a crystalline structure parallel to the fibrous structures of this body. Moreover, mature viral particles were predominantly distributed around the intranuclear inclusion body. Certain viral proteins were also detected in the intranuclear inclusion body by immunoelectron microscopy. We propose that intranuclear inclusion bodies serve as sites of viral replication in HAdV-5-infected cells. Through the 3D analysis, we clarified the relationship between the distribution and localization of intranuclear inclusion bodies and viral particles in HAdV-5-infected cells. These findings can improve our understanding of adenoviral infection mechanisms and may contribute toward developing novel antiviral strategies.

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Cite

BibTeXRIS
BibTeX
@article{wu2026spatiotemporal,
  title = {{Spatiotemporal Analysis of Intranuclear Inclusion Body and Virus Formation in Human Adenovirus Type 5-Infected Cells}},
  author = {Hong Wu and Yoshihiko Fujioka and Shoichi Sakaguchi and Chinami Odake and Youichi Suzuki and Takashi Nakano},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = oct,
  doi = {10.3390/ijms27209181},
  url = {https://www.mdpi.com/1422-0067/27/20/9181},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Spatiotemporal Analysis of Intranuclear Inclusion Body and Virus Formation in Human Adenovirus Type 5-Infected Cells
AU  - Hong Wu
AU  - Yoshihiko Fujioka
AU  - Shoichi Sakaguchi
AU  - Chinami Odake
AU  - Youichi Suzuki
AU  - Takashi Nakano
PY  - 2026
DA  - 2026/10/16
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms27209181
UR  - https://www.mdpi.com/1422-0067/27/20/9181
AB  - Human adenovirus type 5 (HAdV-5) forms a characteristic intranuclear inclusion body in infected cells. However, the relationship between the intranuclear inclusion body and virus formation in HAdV-5-infected cells remains unclear. This study conducted a detailed investigation of the relationship between the structure of the inclusion body and the localization of the virus in HAdV-5-infected cells using electron microscopy and three-dimensional (3D) tomography analytical techniques. Immunostaining for viral proteins was also performed to determine their colocalization with the intranuclear inclusion body. 3D analysis revealed the intranuclear inclusion body as the major site of viral particle accumulation in the cells. Viral particles were identified within the intranuclear inclusion body and arranged in a crystalline structure parallel to the fibrous structures of this body. Moreover, mature viral particles were predominantly distributed around the intranuclear inclusion body. Certain viral proteins were also detected in the intranuclear inclusion body by immunoelectron microscopy. We propose that intranuclear inclusion bodies serve as sites of viral replication in HAdV-5-infected cells. Through the 3D analysis, we clarified the relationship between the distribution and localization of intranuclear inclusion bodies and viral particles in HAdV-5-infected cells. These findings can improve our understanding of adenoviral infection mechanisms and may contribute toward developing novel antiviral strategies.
SN  - 1422-0067
ER  -