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Peer-reviewedCC BYScientific Reports

Visualizing Dolichogenidea gelechiidivoris parasitism within Tuta absoluta larvae through fluorescence microscopy in optically cleared samples

  • Emilio J. Gualda1
  • Alejandro Expósito1
  • Marc Vila-Sala2
  • Pablo Urbaneja-Bernat3
  • Pablo Loza-Álvarez4
  • Jordi Riudavets2
4 affiliations
  1. 1Department of Agri-Food Engineering and Biotechnology (DEAB), Universitat Politècnica de Catalunya
  2. 2Sustainable Plant Protection Program, IRTA
  3. 3Plant Immunity and Biochemistry Group, Biology, Biochemistry and Natural Sciences Department, Universitat Jaume I
  4. 4The Institute of Photonic Sciences (ICFO), Barcelona Institute of Science and Technology
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Abstract

The anatomical studies of host-parasitoid relationships are limited by tissue opacity. Traditionally, this limitation has been overcome using laborious histological dissection techniques. In this work, we present an alternative protocol based on tissue clarification techniques (1-propanol/ECi and 1-propanol/BABB) combined with 3D volumetric fluorescence microscopy (laser scanning confocal microscopy and light sheet fluorescence microscopy). These new protocols allow visualization by autofluorescence not only of the internal anatomy of Tuta absoluta (Lepidoptera: Gelechiidae), but also that of its endoparasitoid Dolichogenidea gelechiidivoris (Hymenoptera: Braconidae). Using this methodology, we were able to identify the three-dimensional position and orientation of D. gelechiidivoris parasitoid within the host as well as its size and morphology at different developmental stages. Due to its minimal invasiveness, which preserves the overall morphological context, these methods, also applicable to other species, will contribute to a deeper understanding of parasitism, providing a basis for future research on host-parasitoid interactions.

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Cite

BibTeXRIS
BibTeX
@article{gualda2026visualizing,
  title = {{Visualizing Dolichogenidea gelechiidivoris parasitism within Tuta absoluta larvae through fluorescence microscopy in optically cleared samples}},
  author = {Emilio J. Gualda and Alejandro Expósito and Marc Vila-Sala and Pablo Urbaneja-Bernat and Pablo Loza-Álvarez and Jordi Riudavets},
  journal = {Scientific Reports},
  year = {2026},
  month = jul,
  doi = {10.1038/s41598-026-62599-9},
  url = {https://doi.org/10.1038/s41598-026-62599-9},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Visualizing Dolichogenidea gelechiidivoris parasitism within Tuta absoluta larvae through fluorescence microscopy in optically cleared samples
AU  - Emilio J. Gualda
AU  - Alejandro Expósito
AU  - Marc Vila-Sala
AU  - Pablo Urbaneja-Bernat
AU  - Pablo Loza-Álvarez
AU  - Jordi Riudavets
PY  - 2026
DA  - 2026/07/01
JO  - Scientific Reports
DO  - 10.1038/s41598-026-62599-9
UR  - https://doi.org/10.1038/s41598-026-62599-9
AB  - The anatomical studies of host-parasitoid relationships are limited by tissue opacity. Traditionally, this limitation has been overcome using laborious histological dissection techniques. In this work, we present an alternative protocol based on tissue clarification techniques (1-propanol/ECi and 1-propanol/BABB) combined with 3D volumetric fluorescence microscopy (laser scanning confocal microscopy and light sheet fluorescence microscopy). These new protocols allow visualization by autofluorescence not only of the internal anatomy of Tuta absoluta (Lepidoptera: Gelechiidae), but also that of its endoparasitoid Dolichogenidea gelechiidivoris (Hymenoptera: Braconidae). Using this methodology, we were able to identify the three-dimensional position and orientation of D. gelechiidivoris parasitoid within the host as well as its size and morphology at different developmental stages. Due to its minimal invasiveness, which preserves the overall morphological context, these methods, also applicable to other species, will contribute to a deeper understanding of parasitism, providing a basis for future research on host-parasitoid interactions.
SN  - 2045-2322
ER  -