Multitargeted antibacterial mechanisms of Iturin A derived from Bacillus amyloliquefaciens against tomato bacterial wilt
4 affiliations
- 1State Tobacco Monopoly Administration (China National Tobacco Corporation), Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences
- 2Hunan Tobacco Research Institute
- 3Enshi Tobacco Science and Technology Center
- 4College of Agriculture, Tarim University
Abstract
Tomato bacterial wilt is a devastating disease caused by Ralstonia solanacearum . This study aimed to evaluate the antibacterial activity of Iturin A, a cyclic lipopeptide produced by Bacillus amyloliquefaciens strain Cas02, against R. solanacearum and investigate its mechanisms of action. The results indicated that Iturin A effectively inhibited the growth of R. solanacearum with an MIC and IC ₅₀ of 25.00 and 15.79 μg/mL, respectively. In pot experiments, Iturin A significantly reduced disease incidence with a control efficacy of 74.27%. Microscopy observations revealed that Iturin A induced severe morphological damage, including membrane disruption and cytoplasmic leakage, and inhibited biofilm formation. Additionally, Iturin A caused the accumulation of intracellular reactive oxygen species, a decrease in succinate dehydrogenase activity, reductions in NAD ⁺ and NADH content, and alterations in cytochrome c oxidase staining, indicating disruption of the electron transport chain and energy metabolism. Transcriptome analysis further indicated the downregulation of genes related to the membrane structure and type III secretion system. Overall, these findings suggest that Iturin A may exert antibacterial effects through multiple pathways, including impairment on cell integrity, biofilm formation, energy metabolism, and virulence-associated functions. This study provides a basis for further exploration of Iturin A as a potential antibacterial agent for managing tomato bacterial wilt.
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BibTeX
@article{yang2026multitargeted,
title = {{Multitargeted antibacterial mechanisms of Iturin A derived from Bacillus amyloliquefaciens against tomato bacterial wilt}},
author = {Jinyu Yang and Tianbo Liu and Zhanjie Li and Tiansheng Zhu and Luping Chi and Xu Yan and Baoxiang Pan and Jinchang Liang and Xiaoqiang Wang},
journal = {Frontiers in Microbiology},
year = {2026},
month = oct,
doi = {10.3389/fmicb.2026.1944894},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2026.1944894/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Multitargeted antibacterial mechanisms of Iturin A derived from Bacillus amyloliquefaciens against tomato bacterial wilt
AU - Jinyu Yang
AU - Tianbo Liu
AU - Zhanjie Li
AU - Tiansheng Zhu
AU - Luping Chi
AU - Xu Yan
AU - Baoxiang Pan
AU - Jinchang Liang
AU - Xiaoqiang Wang
PY - 2026
DA - 2026/10/20
JO - Frontiers in Microbiology
DO - 10.3389/fmicb.2026.1944894
UR - https://www.frontiersin.org/articles/10.3389/fmicb.2026.1944894/full
AB - Tomato bacterial wilt is a devastating disease caused by Ralstonia solanacearum . This study aimed to evaluate the antibacterial activity of Iturin A, a cyclic lipopeptide produced by Bacillus amyloliquefaciens strain Cas02, against R. solanacearum and investigate its mechanisms of action. The results indicated that Iturin A effectively inhibited the growth of R. solanacearum with an MIC and IC ₅₀ of 25.00 and 15.79 μg/mL, respectively. In pot experiments, Iturin A significantly reduced disease incidence with a control efficacy of 74.27%. Microscopy observations revealed that Iturin A induced severe morphological damage, including membrane disruption and cytoplasmic leakage, and inhibited biofilm formation. Additionally, Iturin A caused the accumulation of intracellular reactive oxygen species, a decrease in succinate dehydrogenase activity, reductions in NAD ⁺ and NADH content, and alterations in cytochrome c oxidase staining, indicating disruption of the electron transport chain and energy metabolism. Transcriptome analysis further indicated the downregulation of genes related to the membrane structure and type III secretion system. Overall, these findings suggest that Iturin A may exert antibacterial effects through multiple pathways, including impairment on cell integrity, biofilm formation, energy metabolism, and virulence-associated functions. This study provides a basis for further exploration of Iturin A as a potential antibacterial agent for managing tomato bacterial wilt.
SN - 1664-302X
ER -