Exploring the Antibacterial Activity of Oils Extracted from Phoenix dactylifera (Ajwa and Breem) Seeds Against Multidrug Resistance Bacterial Isolates
- 1Department of Biology, College of Education, Salahaddin University-Erbil, Iraq
- 2Department of Biology, College of Education, Salahaddin University, Erbil, Kurdistan, Iraq
Abstract
Medicinal herbs have historically been used to treat infections, and current research focuses on isolating active plant compounds because of increasing antibiotic resistance and side effects. This study investigates the antimicrobial properties of polyphenol-depleted oil and polyphenol-rich extracts obtained from the seeds of two Phoenix dactylifera (date palm) varieties, Ajwa and Breem. We tested their activity against multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Burkholderia cepacia, Acinetobacter baumannii, Staphylococcus aureus, Streptococcus agalactiae, and Enterococcus faecalis isolates. Antibiotic susceptibility testing showed that most isolates (96%) were resistant to tetracycline, followed by ampicillin and ciprofloxacin (58%). Notably, resistance to critical last-line antibiotics such as imipenem was 10%; colistin, amikacin and moxifloxacin were 8%; and tigecycline was 4%, indicating that resistance remained relatively limited. Antibacterial efficacy of extracted oils of Phoenix dactylifera (date palm) varieties Ajwa and Breem seeds was assessed using both the Minimum inhibitory concentration (MIC) and well diffusion assay. MIC results indicated strong inhibitory potential, ranging from 5 to 10 mg/ml. The well diffusion assay exhibited weaker activity, with inhibition zones reaching up to 13 mm. These results highlight the potential of date seed oil and its polyphenolic components as antimicrobial agents. The study shows that Phoenix dactylifera extracts have promising antimicrobial effects against MDR bacteria. To improve effectiveness, delivery methods such as nanoemulsions or carriers may be needed to enhance the oil’s dispersion and bioavailability.
Related
- Antibacterial efficacy and mechanism of action of VTC3-knockout Saccharomyces cerevisiae against multidrug- resistant pathogenic bacteria
- Editorial: Exploring natural extracts as sustainable antibacterial agents against aquaculture pathogens
- Chemical profiling and in silico evaluation of a Matricaria chamomilla L. extract: exploring its antioxidant, antibacterial, antidiabetic, anti-inflammatory, and anticholinergic activities
- Exploring synergistic effect of bacteriophages with probiotics against multidrug resistant Salmonella Typhimurium in a simulated chicken gastrointestinal system using metagenomic- and culturomic approaches.
- Exploring recurrent urinary tract infection: paired microbiota and culture-based profiling reveals stable dysbiosis and multidrug-resistant, virulence-enriched uropathogens
BibTeX
@article{ismail2026exploring,
title = {{Exploring the Antibacterial Activity of Oils Extracted from Phoenix dactylifera (Ajwa and Breem) Seeds Against Multidrug Resistance Bacterial Isolates}},
author = {Roand muqdad Abdullah Ismail and Payman Akram Hamasaeed},
journal = {Tikrit Journal of Pure Science},
year = {2026},
month = oct,
doi = {10.25130/tjps.v31i5.2063},
url = {https://doi.org/10.25130/tjps.v31i5.2063},
note = {CC BY},
}
RIS
TY - JOUR
TI - Exploring the Antibacterial Activity of Oils Extracted from Phoenix dactylifera (Ajwa and Breem) Seeds Against Multidrug Resistance Bacterial Isolates
AU - Roand muqdad Abdullah Ismail
AU - Payman Akram Hamasaeed
PY - 2026
DA - 2026/10/25
JO - Tikrit Journal of Pure Science
DO - 10.25130/tjps.v31i5.2063
UR - https://doi.org/10.25130/tjps.v31i5.2063
AB - Medicinal herbs have historically been used to treat infections, and current research focuses on isolating active plant compounds because of increasing antibiotic resistance and side effects. This study investigates the antimicrobial properties of polyphenol-depleted oil and polyphenol-rich extracts obtained from the seeds of two Phoenix dactylifera (date palm) varieties, Ajwa and Breem. We tested their activity against multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Burkholderia cepacia, Acinetobacter baumannii, Staphylococcus aureus, Streptococcus agalactiae, and Enterococcus faecalis isolates. Antibiotic susceptibility testing showed that most isolates (96%) were resistant to tetracycline, followed by ampicillin and ciprofloxacin (58%). Notably, resistance to critical last-line antibiotics such as imipenem was 10%; colistin, amikacin and moxifloxacin were 8%; and tigecycline was 4%, indicating that resistance remained relatively limited. Antibacterial efficacy of extracted oils of Phoenix dactylifera (date palm) varieties Ajwa and Breem seeds was assessed using both the Minimum inhibitory concentration (MIC) and well diffusion assay. MIC results indicated strong inhibitory potential, ranging from 5 to 10 mg/ml. The well diffusion assay exhibited weaker activity, with inhibition zones reaching up to 13 mm. These results highlight the potential of date seed oil and its polyphenolic components as antimicrobial agents. The study shows that Phoenix dactylifera extracts have promising antimicrobial effects against MDR bacteria. To improve effectiveness, delivery methods such as nanoemulsions or carriers may be needed to enhance the oil’s dispersion and bioavailability.
SN - 1813-1662
SN - 2415-1726
ER -