Identification of Glycosylation-Associated Proteins in Colostrum: A Review of Mass Spectrometry-Based Strategies.
- 1Laboratory of Proteomics and Microbiology, University of Mons - UMONS, MonsB-7000, Belgium.
Abstract
Colostrum, first milk produced by mammalians after parturition, is a biologically rich and complex fluid, referred to as "liquid gold" due to its exceptional nutritional and immunological properties. Among the bioactive molecules, proteins play critical roles in neonatal development and immune protection, notably due to the presence of glycosylations which support protein folding, stability, recognition, and host-microbe interactions. Thus, glyco-associated proteome characterization in colostrum by mass spectrometry is plebiscite to elucidate its biological functions and therapeutic potential. In this review, we provide a comprehensive overview of MS-based workflows employed for colostrum glycoproteins analysis, highlighting commonly used strategies, methodological specificities, and the gathered biological information. We also discuss alternative analytical approaches that have been successfully applied to colostrum glycoprotein studies. Finally, we present a critical perspective on emerging technologies and unexplored workflows, evaluating their potential applicability, limitations, and future relevance for the analysis of glycosylation-associated proteins in colostrum.
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BibTeX
@article{drewek2026identification,
title = {{Identification of Glycosylation-Associated Proteins in Colostrum: A Review of Mass Spectrometry-Based Strategies.}},
author = {Isalyne Drewek and Blandine Chazarin and Ruddy Wattiez},
journal = {Journal of agricultural and food chemistry},
year = {2026},
month = oct,
doi = {10.1021/acs.jafc.6c06007},
url = {https://europepmc.org/article/MED/42916057},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Identification of Glycosylation-Associated Proteins in Colostrum: A Review of Mass Spectrometry-Based Strategies.
AU - Isalyne Drewek
AU - Blandine Chazarin
AU - Ruddy Wattiez
PY - 2026
DA - 2026/10/01
JO - Journal of agricultural and food chemistry
DO - 10.1021/acs.jafc.6c06007
UR - https://europepmc.org/article/MED/42916057
AB - Colostrum, first milk produced by mammalians after parturition, is a biologically rich and complex fluid, referred to as "liquid gold" due to its exceptional nutritional and immunological properties. Among the bioactive molecules, proteins play critical roles in neonatal development and immune protection, notably due to the presence of glycosylations which support protein folding, stability, recognition, and host-microbe interactions. Thus, glyco-associated proteome characterization in colostrum by mass spectrometry is plebiscite to elucidate its biological functions and therapeutic potential. In this review, we provide a comprehensive overview of MS-based workflows employed for colostrum glycoproteins analysis, highlighting commonly used strategies, methodological specificities, and the gathered biological information. We also discuss alternative analytical approaches that have been successfully applied to colostrum glycoprotein studies. Finally, we present a critical perspective on emerging technologies and unexplored workflows, evaluating their potential applicability, limitations, and future relevance for the analysis of glycosylation-associated proteins in colostrum.
SN - 0021-8561
SN - 1520-5118
ER -