Genome-Wide Characterization and Multidimensional Transcriptome Profiling of the SABATH Methyltransferase Gene Family in Sunflower (Helianthus annuus L.)
- 1Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Key Laboratory of Cotton Biology and Genetic Breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Hebei Key Laboratory of Cotton Bio-Breeding and Cultivation Physiology, Shijiazhuang 050051, China
Abstract
Plant SABATH methyltransferases contribute to volatile formation, hormone homeostasis and stress responses, but their family-wide organization in sunflower remains poorly characterized. Using the HanXRQr2.0-SUNRISE reference genome, we identified 33 high-confidence HaSABATH genes and integrated phylogenetic, duplication, synteny, promoter and protein-structure analyses with expression profiles from 200 public RNA sequencing (RNA-seq) libraries representing 87 distinct conditions. Twelve genes belonged to the salicylic acid/jasmonic acid methyltransferase-related Clade I, 19 to the benzoic acid/salicylic acid methyltransferase-related Clade II, and two to other lineages. Tandem, proximal, dispersed and whole-genome/segmental duplication contributed to the family, with overlapping tandem and segmental evidence for HaSABATH4 and HaSABATH15. Eleven reliable nonsynonymous-to-synonymous substitution ratios were below one. Most predicted proteins showed high model confidence. Across the sampled conditions, Clade I members generally showed higher expression levels and broader expression, while many Clade II members were weakly expressed or condition-biased. HaSABATH4 and HaSABATH6 were prioritized for follow-up under combined saline–alkali stress. This integrated analysis links evolutionary context to expression divergence and provides candidates for targeted biochemical and genetic studies.
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BibTeX
@article{gao2026genomewide,
title = {{Genome-Wide Characterization and Multidimensional Transcriptome Profiling of the SABATH Methyltransferase Gene Family in Sunflower (Helianthus annuus L.)}},
author = {Xinlong Gao and Kaihui Wang and Mengzhe Li and Dan Li and Xu Liu and Jiangna Zheng and Suen Liu and Junyi Geng and Baosheng Guo and Yang Han and Haina Zhang and Cunpeng Zhao},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27219805},
url = {https://www.mdpi.com/1422-0067/27/21/9805},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Genome-Wide Characterization and Multidimensional Transcriptome Profiling of the SABATH Methyltransferase Gene Family in Sunflower (Helianthus annuus L.)
AU - Xinlong Gao
AU - Kaihui Wang
AU - Mengzhe Li
AU - Dan Li
AU - Xu Liu
AU - Jiangna Zheng
AU - Suen Liu
AU - Junyi Geng
AU - Baosheng Guo
AU - Yang Han
AU - Haina Zhang
AU - Cunpeng Zhao
PY - 2026
DA - 2026/10/31
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27219805
UR - https://www.mdpi.com/1422-0067/27/21/9805
AB - Plant SABATH methyltransferases contribute to volatile formation, hormone homeostasis and stress responses, but their family-wide organization in sunflower remains poorly characterized. Using the HanXRQr2.0-SUNRISE reference genome, we identified 33 high-confidence HaSABATH genes and integrated phylogenetic, duplication, synteny, promoter and protein-structure analyses with expression profiles from 200 public RNA sequencing (RNA-seq) libraries representing 87 distinct conditions. Twelve genes belonged to the salicylic acid/jasmonic acid methyltransferase-related Clade I, 19 to the benzoic acid/salicylic acid methyltransferase-related Clade II, and two to other lineages. Tandem, proximal, dispersed and whole-genome/segmental duplication contributed to the family, with overlapping tandem and segmental evidence for HaSABATH4 and HaSABATH15. Eleven reliable nonsynonymous-to-synonymous substitution ratios were below one. Most predicted proteins showed high model confidence. Across the sampled conditions, Clade I members generally showed higher expression levels and broader expression, while many Clade II members were weakly expressed or condition-biased. HaSABATH4 and HaSABATH6 were prioritized for follow-up under combined saline–alkali stress. This integrated analysis links evolutionary context to expression divergence and provides candidates for targeted biochemical and genetic studies.
SN - 1422-0067
ER -