Distinct In Vivo Electrophysiological Profiles of Mediodorsal Thalamus Subdivisions
- 1University of Oxford
Abstract
The mediodorsal nucleus of the thalamus (MD) plays a key role in complex cognitive processes, and its dysfunction is linked to various neurological and psychiatric conditions. The MD is divided into three parts that vary in anatomical connectivity, molecular expression, and ex vivo electrophysiology. Here, we describe in vivo single-neuron electrophysiological recordings across the three MD subdivisions in head-fixed, behaving, adult, wildtype mice. We report large differences in extracellular waveforms, spiking activity, and burst firing characteristics across MD subdivisions. Specifically, central MD subdivision (MDc) neurons showed markedly increased waveform amplitude, higher spontaneous firing rate, and increased burst firing compared to medial (MDm) and lateral (MDl) subdivision neurons. Single-neuron electrophysiology features were sufficient to classify neurons into respective anatomical subdivisions. Hierarchical clustering revealed MDc is distinct from MDl and MDm and more akin to other, non-MD thalamic nuclei. Together, these data suggest distinct in vivo electrophysiological profiles of MD subdivisions, posing implications for investigating MD thalamus in health and disease.
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BibTeX
@misc{panti2026distinct,
title = {{Distinct In Vivo Electrophysiological Profiles of Mediodorsal Thalamus Subdivisions}},
author = {K. Panti and C. J. Stagg and A. Sharott and J. Stedehouder},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.02.748524},
url = {https://www.biorxiv.org/content/10.64898/2026.09.02.748524v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Distinct In Vivo Electrophysiological Profiles of Mediodorsal Thalamus Subdivisions
AU - K. Panti
AU - C. J. Stagg
AU - A. Sharott
AU - J. Stedehouder
PY - 2026
DA - 2026/09/02
PB - bioRxiv
DO - 10.64898/2026.09.02.748524
UR - https://www.biorxiv.org/content/10.64898/2026.09.02.748524v1
AB - The mediodorsal nucleus of the thalamus (MD) plays a key role in complex cognitive processes, and its dysfunction is linked to various neurological and psychiatric conditions. The MD is divided into three parts that vary in anatomical connectivity, molecular expression, and ex vivo electrophysiology. Here, we describe in vivo single-neuron electrophysiological recordings across the three MD subdivisions in head-fixed, behaving, adult, wildtype mice. We report large differences in extracellular waveforms, spiking activity, and burst firing characteristics across MD subdivisions. Specifically, central MD subdivision (MDc) neurons showed markedly increased waveform amplitude, higher spontaneous firing rate, and increased burst firing compared to medial (MDm) and lateral (MDl) subdivision neurons. Single-neuron electrophysiology features were sufficient to classify neurons into respective anatomical subdivisions. Hierarchical clustering revealed MDc is distinct from MDl and MDm and more akin to other, non-MD thalamic nuclei. Together, these data suggest distinct in vivo electrophysiological profiles of MD subdivisions, posing implications for investigating MD thalamus in health and disease.
ER -