Time-series reconstruction of the molecular architecture of human centriole assembly
Abstract
Centriole biogenesis, as in most organelle assemblies, involves the sequential recruitment of sub-structural elements that will support its function. To uncover this process, we correlated the spatial location of 24 centriolar proteins with structural features using expansion microscopy. A time-series reconstruction of protein distributions throughout human procentriole assembly unveiled the molecular architecture of the centriole biogenesis steps. We found that the process initiates with the formation of a naked cartwheel devoid of microtubules. Next, the bloom phase progresses with microtubule blade assembly, concomitantly with radial separation and rapid cartwheel growth. In the subsequent elongation phase, the tubulin backbone grows linearly with the recruitment of the A-C linker, followed by proteins of the inner scaffold (IS). By following six structural modules, we modeled 4D assembly of the human centriole. Collectively, this work provides a framework to investigate the spatial and temporal assembly of large macromolecules.
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BibTeX
@article{laporte2026timeseries,
title = {{Time-series reconstruction of the molecular architecture of human centriole assembly}},
author = {Marine Laporte and Davide Gambarotto and Éloïse Bertiaux and Lorène Bournonville and Vincent Louvel and José Nunes and Susanne Borgers and Virginie Hamel and Paul Guichard},
journal = {Cell},
year = {2026},
month = sep,
doi = {10.1016/j.cell.2024.03.025},
url = {https://hal.science/hal-05760463v1},
note = {CC BY-NC},
}
RIS
TY - JOUR
TI - Time-series reconstruction of the molecular architecture of human centriole assembly
AU - Marine Laporte
AU - Davide Gambarotto
AU - Éloïse Bertiaux
AU - Lorène Bournonville
AU - Vincent Louvel
AU - José Nunes
AU - Susanne Borgers
AU - Virginie Hamel
AU - Paul Guichard
PY - 2026
DA - 2026/09/23
JO - Cell
DO - 10.1016/j.cell.2024.03.025
UR - https://hal.science/hal-05760463v1
AB - Centriole biogenesis, as in most organelle assemblies, involves the sequential recruitment of sub-structural elements that will support its function. To uncover this process, we correlated the spatial location of 24 centriolar proteins with structural features using expansion microscopy. A time-series reconstruction of protein distributions throughout human procentriole assembly unveiled the molecular architecture of the centriole biogenesis steps. We found that the process initiates with the formation of a naked cartwheel devoid of microtubules. Next, the bloom phase progresses with microtubule blade assembly, concomitantly with radial separation and rapid cartwheel growth. In the subsequent elongation phase, the tubulin backbone grows linearly with the recruitment of the A-C linker, followed by proteins of the inner scaffold (IS). By following six structural modules, we modeled 4D assembly of the human centriole. Collectively, this work provides a framework to investigate the spatial and temporal assembly of large macromolecules.
SN - 0092-8674
SN - 1097-4172
ER -