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Peer-reviewedCC BY 4.0Frontiers in Physiology

Sampling matters: the interplay of physiological parameters and performance over 1, 2 and 3 km in well-trained male endurance runners and sprinters

4 affiliations
  1. 1Institute of Sports and Sports Science, Karlsruhe Institute of Technology, Karlsruhe, Germany
  2. 2Institute of Movement and Neurosciences, German Sport University Cologne, Cologne, Germany
  3. 3Exercise Science, Coaching and Performance Enhancement (ESCAPE) Network, Cologne, Germany
  4. 4Institute of Exercise Training and Sport Informatics, German Sport University Cologne, Cologne, Germany

Abstract

Aim: To examine characteristics associated with running performance in heterogeneous and endurance-only samples of well-trained male runners.

Methods: Twenty well-trained (V̇O2max: 65.7 ± 5.5 ml·min-1·kg-1) male endurance runners (n=16) and sprinters (n=4), completed comprehensive laboratory testing and 100-m sprint as well as, 1-, 2-, and 3-km time-trials. Physiological markers included fractional utilization at maximal lactate steady-state (%V̇O2max), maximal fat oxidation rate (MFO), maximal lactate accumulation rate (ċLamax) and peak post-exercise lactate following the ramp test (LaRT). Hierarchical regression and bivariate correlations with Benjamini-Hochberg correction were used to examine performance associations and the influence of excluding sprinters.

Results: Baseline models (V̇O2max + running economy) explained <40% of 1000-m and >60% in 2000- and 3000-m performance in the pooled sample. MFO increased explained variance by 15.4% at 2000 m and +14.81% at 3000 m, while %V̇O2max added 11.3% at 3000 m. In the pooled sample, oxidative characteristics became more strongly associated with performance as distance increased, whereas several non-oxidative characteristics were positively associated with sprint performance and negatively associated with longer-distance performance. After excluding sprinters, associations of V̇O2max and MFO with 2000- and 3000-m performance were retained, whereas associations involving ċLamax and LaRT were attenuated. ASR remained negatively associated with 3000-m performance in both samples.

Conclusion: In well-trained male runners, associations between physiological characteristics and running performance depend on event distance and sample composition. Oxidative characteristics were particularly relevant to 2000- and 3000-m performance, whereas the apparent relevance of several non-oxidative characteristics partly reflected the inclusion of sprinters. These sampling-related findings require confirmation in larger, balanced cohorts.

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BibTeX
@article{sondermann2026sampling,
  title = {{Sampling matters: the interplay of physiological parameters and performance over 1, 2 and 3 km in well-trained male endurance runners and sprinters}},
  author = {Yannick M. Sondermann and Oliver J. Quittmann and Simon Nolte and Marco Fuchs and Gabriel Gehlert and Tina Foitschik and Thomas Abel},
  journal = {Frontiers in Physiology},
  year = {2026},
  month = oct,
  doi = {10.3389/fphys.2026.1912310},
  url = {https://doi.org/10.3389/fphys.2026.1912310},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Sampling matters: the interplay of physiological parameters and performance over 1, 2 and 3 km in well-trained male endurance runners and sprinters
AU  - Yannick M. Sondermann
AU  - Oliver J. Quittmann
AU  - Simon Nolte
AU  - Marco Fuchs
AU  - Gabriel Gehlert
AU  - Tina Foitschik
AU  - Thomas Abel
PY  - 2026
DA  - 2026/10/22
JO  - Frontiers in Physiology
DO  - 10.3389/fphys.2026.1912310
UR  - https://doi.org/10.3389/fphys.2026.1912310
AB  - Aim: To examine characteristics associated with running performance in heterogeneous and endurance-only samples of well-trained male runners.  Methods: Twenty well-trained (V̇O2max: 65.7 ± 5.5 ml·min-1·kg-1) male endurance runners (n=16) and sprinters (n=4), completed comprehensive laboratory testing and 100-m sprint as well as, 1-, 2-, and 3-km time-trials. Physiological markers included fractional utilization at maximal lactate steady-state (%V̇O2max), maximal fat oxidation rate (MFO), maximal lactate accumulation rate (ċLamax) and peak post-exercise lactate following the ramp test (LaRT). Hierarchical regression and bivariate correlations with Benjamini-Hochberg correction were used to examine performance associations and the influence of excluding sprinters.  Results: Baseline models (V̇O2max + running economy) explained <40% of 1000-m and >60% in 2000- and 3000-m performance in the pooled sample. MFO increased explained variance by 15.4% at 2000 m and +14.81% at 3000 m, while %V̇O2max added 11.3% at 3000 m. In the pooled sample, oxidative characteristics became more strongly associated with performance as distance increased, whereas several non-oxidative characteristics were positively associated with sprint performance and negatively associated with longer-distance performance. After excluding sprinters, associations of V̇O2max and MFO with 2000- and 3000-m performance were retained, whereas associations involving ċLamax and LaRT were attenuated. ASR remained negatively associated with 3000-m performance in both samples.  Conclusion: In well-trained male runners, associations between physiological characteristics and running performance depend on event distance and sample composition. Oxidative characteristics were particularly relevant to 2000- and 3000-m performance, whereas the apparent relevance of several non-oxidative characteristics partly reflected the inclusion of sprinters. These sampling-related findings require confirmation in larger, balanced cohorts.
SN  - 1664-042X
ER  -